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Plasmid#193782PurposeExpress TadCBEa (with SpCas9) in mammalian cellsDepositorInsertTadA-CDa-SpCas9D10A
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFP-SspBx6
Plasmid#103778Purposeexpression of iPOLYMER component in mammalian cellsDepositorInsertYFP-SspBx6
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(C163S)
Plasmid#90088PurposeExpresses caspase 3 with C163S to prevent autocatalysis in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationC163SPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-N-mEGFP-PARP15
Plasmid#178000PurposeExpresses N-terminally mEGFP-tagged PARP15DepositorInsertPARP15 (PARP15 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPUR-mU6-sgRNA-2XPP7
Plasmid#194504PurposeEmpty backbone for sgRNA-2XPP7 in CRIPSR FISHer systemDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
SSB-wt
Plasmid#197260Purposewild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistanceDepositorInsertwt E. coli SSB
UseTagsnoneExpressionBacterialMutationPromoterT7Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAV1-K*R-mEGFP
Plasmid#27766DepositorInsertcaveolin-1, lysine null mutant (CAV1 Human)
UseTagsmonomeric EGFPExpressionMammalianMutationAll lysines mutated to arginines (K*R)PromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II KS(+) ACMV DNA-A 1.4mer
Plasmid#159134PurposeInfectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-ADepositorInsertACMV DNA-A 1.4mer
UseInfectious clone for bombarding plantsTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2 EGFP sgRNA2
Plasmid#164933PurposeExpresses EGFP sgRNA2 in mammalian cellsDepositorInsertsgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
UseLentiviralTagsExpressionMutationPromoterEFS promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Akt-STOPS-NES
Plasmid#175008PurposeCytosol-targeted Akt-STOPS.DepositorInsertmCherry-Akt-STOPS-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBUN421
Plasmid#62204PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterTaU3 and UbiAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-NES
Plasmid#118939PurposeUsed to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
1127-DP NTP wild type
Plasmid#32398DepositorInsertDesmoplakin (DSP Human)
UseTagsFLAGExpressionMammalianMutationContains only the first 584 aaPromoterCMVAvailable sinceNov. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAEL010097-Cas9
Plasmid#100707PurposeThe promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL010097-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL010097 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
Plasmid#71719PurposeExpressing a GFP-tagged degron in the soma of C. elegansDepositorInsertauxin-responsive protein IAA17 (AXR3 Mustard Weed)
UseTagsEmGFPExpressionWormMutationminimal degron sequence (71-114aa) with start cod…Promotereef-1A.1 (eft-3)Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
nLuc-ΔECD-PTHR1
Plasmid#190011PurposeExpresses the Nanoluciferase fused to the human parathyroid hormone receptor type 1 that is missing the extracellular domainDepositorInsertnLuc-ΔECD-PTHR1 (PTH1R Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLYS1-EMRE/RNAi resistant
Plasmid#50056PurposeExpression of human EMRE in mammalian cells with silent mutation making it resistant to RNAiDepositorInsertEMRE-RNAi resistant (SMDT1 Human)
UseTagsExpressionMammalianMutationRNAi resistant, silent mutantPromoterAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Th3
Plasmid#188064PurposePROSS-designed high-redox potential laccase from Trametes hirsutaDepositorInsertPROSS-designed high-redox potential laccase from Trametes hirsuta
UseTagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation20 mutations, described in publicationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Tv9nL
Plasmid#188069PurposePROSS-designed high-redox potential laccase from Trametes versicolorDepositorInsertPROSS-designed high-redox potential laccase from Trametes versicolor
UseTagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation79 PROSS mutations, described in publicationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only