We narrowed to 9,184 results for: Pol;

1. pGEMHE-bNup98-DN-NLS-mClover3

   Plasmid

   #171495

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   Nup98-DN (NUP98 B. tarus (bovine))
   

   Use

   Pgehme

   Tags

   mClover3

   Promoter

   T7

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV BARK R106A p2A mCherry

   Plasmid

   #117688

   Purpose

   CMV-iBARK(R106A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs R106A peptide
   

   Tags

   HA / FLAG

   Expression

   Mammalian

   Mutation

   R106A

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CPH3566 (pDEST15_Spt6 1223-1451)

   Plasmid

   #91814

   Purpose

   Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deleted residues 1-1222

   Promoter

   T7

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CPH1141 (pET151_Spt6 1247-1451)

   Plasmid

   #91815

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   deleted residues 1-1246

   Promoter

   T7

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p1A CbbL-

   Plasmid

   #162693

   Purpose

   p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   H. neapolitanus CbbLS and S. elongatus Prk
   

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Mutation

   CbbL K194M (inactive rubisco)

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNG1304

   Plasmid

   #162488

   Purpose

   Expresses HelD K239A from Bacillus subtillis

   Depositor

   Peter Lewis

   Article

   Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

   Insert

   HelD (helD Bacillus subtillis 168)
   

   Tags

   6His-V5

   Expression

   Bacterial

   Mutation

   Changed Lysine 239 to Alanine (K239A)

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_gRNA_IG-DMR-1

   Plasmid

   #159931

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, CBh

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330_gRNA_IG-DMR-2

   Plasmid

   #159932

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, CBh

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-Basic-ZC3H12D_PromoterWT

   Plasmid

   #153065

   Purpose

   ZC3H12D promoter region cloned upstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   ZC3H12D promoter region (ZC3H12D Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   None

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKM5

    Plasmid

    #154166

    Purpose

    Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel2 (long isoform)
    

    Tags

    FLAG

    Expression

    Insect

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKM28

    Plasmid

    #154165

    Purpose

    Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel1A
    

    Tags

    FLAG

    Expression

    Insect

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-Mm_Plk5(D168A)

    Plasmid

    #136318

    Purpose

    Mammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (Plk5 Mouse)
    

    Expression

    Mammalian

    Mutation

    D168A

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Hu_Plk5(W207F)

    Plasmid

    #136320

    Purpose

    Mammalian expression of human Plk5 W207F with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (PLK5 Human)
    

    Expression

    Mammalian

    Mutation

    W207F

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-XYLT2-sgRNA

    Plasmid

    #154862

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting XYLT2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    XYLT2 sgRNA (XYLT2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDF11-SFPQ(214-535)

    Plasmid

    #135439

    Purpose

    Expresses SFPQ 214-535 with TEV-cleavable hexahistidine tag

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    splicing factor proline and glutamine rich (SFPQ Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET14b-dabAB2 D353A

    Plasmid

    #133016

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    D353A

    Promoter

    t7

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET14b-dabAB2 C351A

    Plasmid

    #133014

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    C351A

    Promoter

    t7

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15b-GreBdm-D41N

    Plasmid

    #129691

    Purpose

    express E. coli GreB E82C/C68S/D41N

    Depositor

    Jeff Gelles

    Article

    Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GreB E82C C68S D41N (greB E. coli)
    

    Tags

    his6

    Expression

    Bacterial

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMH-HT_CtCHAD-H253A-R256A-R260A

    Plasmid

    #127788

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A

    Plasmid

    #127789

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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