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pDONR221-W279A-short-UBASH3A-Ctag

Plasmid

#195402

Purpose

Human W279A short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Gateway donor vector

Mutation

W279A

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-K202R-short-UBASH3A-Ctag

Plasmid

#195399

Purpose

Human K202R short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Gateway donor vector

Mutation

K202R

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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K202R-short-UBASH3A-V5-6xHis

Plasmid

#195406

Purpose

Mammalian expression of human K202R short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5-6xHis

Expression

Mammalian

Mutation

K202R

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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W279A-short-UBASH3A-V5-6xHis

Plasmid

#195409

Purpose

Mammalian expression of human W279A short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5-6xHis

Expression

Mammalian

Mutation

W279A

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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V5-K202R-short-UBASH3A

Plasmid

#192084

Purpose

Mammalian expression of human K202R short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5

Expression

Mammalian

Mutation

K202R

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-W279A-short-UBASH3A-Ntag

Plasmid

#192096

Purpose

Human W279A short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Mutation

W279A

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-K202R-short-UBASH3A-Ntag

Plasmid

#192094

Purpose

Human K202R short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Mutation

K202R

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-WT-short-UBASH3A-Ctag

Plasmid

#192099

Purpose

Human wild-type short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-WT-short-UBASH3A-Ntag

Plasmid

#192100

Purpose

Human wild-type short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pHis8-4b-McDAH

Plasmid

#140129

Purpose

Expresses McDAH with N-terminal 8xHis-tag in BL21 E. coli

Depositor

Jing-Ke Weng

Article

Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

Insert

Dechloroacutumine halogenase from Menispermum canadense

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pOZ-FH-C Bach1 K141A/K142A

Plasmid

#73646

Purpose

Retroviral expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)

Depositor

Sharon Cantor

Insert

Bach1 (BACH1 Human)

Use

Retroviral

Tags

FLAG and His

Expression

Mammalian

Mutation

K141A/K142A ( mlh1 binding mutant)

Promoter

unknown

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only
pCP-tRNA

Plasmid

#133812

Purpose

CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

Depositor

Geraldine Butler

Article

Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

Insert

cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter

Use

CRISPR

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG SMG1 WT

Plasmid

#199573

Purpose

Expresses FLAG-tagged WT SMG1

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRFHUE-eGFP

Plasmid

#89469

Purpose

GFP labelling of filamentous fungi by Agrobacterium tumefaciens mediated transformation

Depositor

Luis González-Candelas

Article

Crespo-Sempere et al Int J Food Microbiol. 2011 Aug 2;148(2):

Insert

eGFP

Use

Binary plasmid for atmt of filamentous fungi

Promoter

A. nidulans gpdA

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-CBLB

Plasmid

#192082

Purpose

Mammalian expression of human CBLB with FLAG tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

CBLB (CBLB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-FLAG-LKB1

Plasmid

#8592

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

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GFP SMG1 WT

Plasmid

#199571

Purpose

Expresses WT SMG1 fused to GFP

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 WT (SMG1 Human)

Tags

GFP fusion protein

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK2-3xFLAG

Plasmid

#233895

Purpose

lentiviral overexpression vector for NADK2

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

NADK2 (NADK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

Ubc

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-Blast-SHMT2-3xFLAG

Plasmid

#233891

Purpose

lentiviral overexpression vector for SHMT2

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

SHMT2 (SHMT2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK-3xFLAG

Plasmid

#163452

Purpose

lentiviral overexpression vector for human NADK

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

NADK (NADK Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available Since

March 4, 2021

Availability

Academic Institutions and Nonprofits only

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