Purpose
S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).
Mutation
ORF for Nip1 encodes aa 4-156 (not complete ORF),…
Available Since
Jan. 6, 2020
Availability
Academic Institutions and Nonprofits only