We narrowed to 6,950 results for: SIM

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pInducer10-SAE1_sh3

Plasmid

#168985

Purpose

Expresses RFP cDNA and SAE1 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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MSCVhyg-HA-FLAG_Ubc9_C93A

Plasmid

#164937

Purpose

Expresses FLAG & HA tagged human Ubiquitin Conjugating Enzyme 9 (UBC9) _C93A mutant cDNA

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

human UBC9 (UBE2I Human)

Use

Retroviral

Tags

FLAG & HA tags

Promoter

PGK promoter

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-OLIG2-Adel-IDR

Plasmid

#145249

Purpose

Expresses fusion of GAL4 DNA-binding domain and OLIG2-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

OLIG2-Adel-IDR (OLIG2 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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R979-X57-008: Hs.KRAS4b(1-169)

Plasmid

#159690

Purpose

Baculovirus protein expression of KRAS4b (1-169)

Depositor

Dominic Esposito

Article

Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

Insert

Hs.KRAS4b(1-169) (KRAS Human)

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-TBP(38Q)-IDR-mCherry

Plasmid

#145283

Purpose

Bacterial Expression of 6xHis TBP(38Q) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

TBP(38Q)-IDR (TBP Human)

Expression

Bacterial

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(DEdel)-IDR-mCherry-CRY2-NLS

Plasmid

#145273

Purpose

Mammalian Expression of Nuclear Opto HOXD13(DEdel) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(D/E del)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

D/E deleted

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(-7A)-IDR-mCherry-CRY2-NLS

Plasmid

#145271

Purpose

Mammalian Expression of Nuclear Opto HOXD13(-7A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(-7A)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

A-repeat deleted

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXA13(+7A)-IDR-mCherry-CRY2-NLS

Plasmid

#145266

Purpose

Mammalian Expression of Nuclear Opto HOXA13(+7A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13(+7A)-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXA13-IDR-mCherry-CRY2-NLS

Plasmid

#145265

Purpose

Mammalian Expression of Nuclear Opto HOXA13 IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13wt-IDR (HOXA13 Human)

Expression

Mammalian

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD13-Adel-15A-IDR

Plasmid

#145243

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXD13-Adel-15A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13-Adel-15A-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13-44A-IDR

Plasmid

#145237

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13-44A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13-44A-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13-15A-IDR

Plasmid

#145236

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13-15A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13-15A-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HEY2-Adel-IDR

Plasmid

#145234

Purpose

Expresses fusion of GAL4 DNA-binding domain and HEY2-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HEY2-Adel-IDR (HEY2 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-BHLHE41-Adel-IDR

Plasmid

#145228

Purpose

Expresses fusion of GAL4 DNA-binding domain and BHLHE41-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

BHLHE41-Adel-IDR (BHLHE41 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3e_NAB

Plasmid

#156473

Purpose

Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3e_NAB (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc [ArchT-tdTomato]

Plasmid

#123607

Purpose

AAV mediated expression of ArchT-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. tdTomato has codons varied to reduce recombination. Using bGHpA signal.

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Promoter

Syn

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-VRER-P2A-GFP-PGK-Puro

Plasmid

#110862

Purpose

Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRER

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-P2A-GFP-PGK-Puro

Plasmid

#110863

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLuc-Optop38i-lsm

Plasmid

#89747

Purpose

Expression of light-regulated p38 inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i lit-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

I539E mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLuc-OptoJNKi-lsm

Plasmid

#89746

Purpose

Expression of light-regulated JNK inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi lit-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

I539E mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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