We narrowed to 14,477 results for: nts

1. pDGE334

   Plasmid

   #153243

   Purpose

   M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

   Plasmid

   #175523

   Purpose

   Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   LAMP1-HaloTag11 and Tomm20-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-hAsCpf1(TATV) (pY220)

   Plasmid

   #89353

   Purpose

   Expresses humanized AsCpf1 variant that recognizes TATV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RVR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K548V/N552R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMOD_B2520

   Plasmid

   #91077

   Purpose

   Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Promoter

   OsU6

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-DIO-EYFP

   Plasmid

   #104052

   Purpose

   An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Has Service

   AAV PHP.V1

   Insert

   EYFP
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB452

   Plasmid

   #74834

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mH2A1.1-CT-GFP

   Plasmid

   #45167

   Depositor

   Brian Chadwick, Hunt Willard

   Article

   Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

   Insert

   H2Afy (MACROH2A1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. xCas9(3.7)-BE3

   Plasmid

   #108380

   Purpose

   Mammalian expression vector for xCas9(3.7)-BE3

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.7)-BE3
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiPGK Hygro DEST H2B-mCerulean3

   Plasmid

   #90234

   Purpose

   Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   H2B
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB433

    Plasmid

    #74824

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDR111_Bgal no NLS

    Plasmid

    #188397

    Purpose

    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Promoter

    Phyper spank

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP Rootletin (Nigg pFL2(CW499))

    Plasmid

    #41166

    Depositor

    Erich Nigg

    Article

    Bahe et al J Cell Biol. 2005 Oct 10;171(1):27-33. E

    Insert

    Rootletin (CROCC Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBiFC-bJunVN173

    Plasmid

    #22012

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    bJunVN173 (Jun Aequorea Victoria, Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    c-Jun(257-338)-Venus(1-172)

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB411

    Plasmid

    #74805

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJAB988

    Plasmid

    #133920

    Purpose

    miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter

    Depositor

    Christopher Voigt

    Article

    Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.

    Insert

    miniICE-tet(M)cat(PC194)_PT7-GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRIS

    Plasmid

    #120424

    Purpose

    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.

    Depositor

    Tamás Fehér

    Article

    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

    Insert

    CRISPR spacers targeting IS1, IS5, IS3, IS150
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    constitutive

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGM55261

    Plasmid

    #153210

    Purpose

    Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Phi3-GCaMP6f

    Plasmid

    #106298

    Purpose

    CMV driven GFP based calcium (Ca2+) reporter

    Depositor

    Sanford Simon

    Article

    Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

    Insert

    GCaMP6f
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

    Plasmid

    #175525

    Purpose

    Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10 and Tomm20-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA-3xHA-Reptin

    Plasmid

    #51636

    Purpose

    Mamamlian expression of 3xHA tagged reptin, N-terminal.

    Depositor

    Steven Artandi

    Article

    Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

    Insert

    reptin (RUVBL2 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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