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pLPC-Flag-CHES1L-dFN

Plasmid

#51842

Purpose

Flag-CHES1L mutant lacking the forkhead binding site and N-terminal domains

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Mutation

contains CHES1 amino acids 206-490

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1L-dC

Plasmid

#51839

Purpose

Flag-CHES1L mutant lacking the C-terminal and NLS domains

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Mutation

contains CHES1 amino acids 1-205

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1L-dN

Plasmid

#51843

Purpose

Flag-CHES1L mutant lacking the N-terminal domain

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Mutation

contains CHES1 amino acids 110-490

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1L-DBD-NLS

Plasmid

#51841

Purpose

Flag-CHES1L mutant encoding the DNA binding and NLS domains only

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag and NLS

Mutation

contains CHES1 amino acids 110-205

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1L-dC-NLS

Plasmid

#51840

Purpose

Flag-CHES1L mutant lacking the C-terminal, with remaining NLS

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag and NLS

Mutation

contains CHES1 amino acids 1-205

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1LdFC-NLS

Plasmid

#51838

Purpose

Flag-CHES1L mutant lacking the forkhead binding site and C-terminal domain, with remaining NLS

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag and NLS

Mutation

contains CHES1 amino acids 1-109

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES11L-dFC

Plasmid

#51837

Purpose

Flag-CHES1L mutant lacking the forkhead binding site and the C-terminal domains

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Mutation

contains CHES1 amino acids 1-109

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-Delta-ATG1-mMCL1

Plasmid

#45823

Purpose

This plasmid was made to show that the two MCL-1 bands DO NOT arise from a non-canonical translation initiation downstream of the start codon.

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

Delta ATG1-mMCL1

Expression

Mammalian

Mutation

The first amino acid (ATG) is deleted from the fu…

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT

Plasmid

#111072

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 BD (522-645, C643A)/pET28HMT

Plasmid

#111073

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 BD (522-645, C643A)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.5 AB (361-545, Δ395-511)/pET28HMT

Plasmid

#111267

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.5 AB (361-545, Δ395-511)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-645, Δ368-492)/pcDNA3.1

Plasmid

#111454

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-492), C643A

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-645, Δ368-523)/I346A/HA tag/pcDNA3.1

Plasmid

#111460

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

Ha Tag

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-523)/I346A, C643A

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1

Plasmid

#111461

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

Ha Tag

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-523)/I346D, C643A

Availability

Academic Institutions and Nonprofits only

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mRFP-Rab5

Plasmid

#14437

Purpose

Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

Depositor

Ari Helenius

Article

Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

Insert

Rab 5 (RAB5A Human)

Tags

mRFP

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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mRFP-Rab7

Plasmid

#14436

Purpose

Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

Depositor

Ari Helenius

Article

Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

Insert

Rab 7 (RAB7A Canis familialis)

Tags

mRFP

Expression

Mammalian

Mutation

3 silent mutations near the C-terminus

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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GFP-EEA1 wt

Plasmid

#42307

Depositor

Silvia Corvera

Article

Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

Insert

EEA1 (EEA1 Human)

Tags

GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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NM9-SpCas9-NLS3

Plasmid

#128177

Purpose

Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

SpCas9-NLS3

Use

CRISPR

Expression

Yeast

Promoter

pPGK1

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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NM1-5S-tRNA-SgH

Plasmid

#128178

Purpose

Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

gRNA cloning cassette

Use

CRISPR

Expression

Yeast

Promoter

5S rRNA-tRNA(Arg)

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C-Halo

Plasmid

#195153

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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