165,588 results

1. pYPQAT51

   Plasmid

   #223423

   Purpose

   T-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-LbCas12a-RRV-2x35s-crRNA scaffold
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. mVenus C1

   Plasmid

   #27794

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Has Service

   Cloning Grade DNA

   Insert

   mVenus C1
   

   Expression

   Mammalian

   Available Since

   April 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458-mcherry

   Plasmid

   #161974

   Purpose

   Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

   Depositor

   Joanna Wysocka

   Article

   Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

   Insert

   Cas9-t2a-mcherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

   Plasmid

   #213030

   Purpose

   Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
   

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. anti-FLAG M2 heavy chain

   Plasmid

   #175359

   Purpose

   Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

   Depositor

   Joost Snijder

   Article

   Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

   Insert

   anti-FLAG M2 heavy chain
   

   Tags

   His8 tag and N-terminal secretion signal peptide …

   Expression

   Mammalian

   Mutation

   L51I (see paper)

   Promoter

   CMV

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIRT2 Flag

   Plasmid

   #13813

   Purpose

   Mammalian expression of human SIRT2 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT2 (SIRT2 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Transcript variant 2

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 phiC31

   Plasmid

   #68310

   Purpose

   In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

   Depositor

   Konrad Basler

   Article

   Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

   Insert

   phiC31
   

   Use

   Rna transcription

   Promoter

   CMV

   Available Since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdCas9-bacteria

   Plasmid

   #44249

   Purpose

   aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 (bacteria)
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   pLtetO-1

   Available Since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-DIO-hM3D(Gq)-mCherry

   Plasmid

   #50460

   Purpose

   Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   hM3D(Gq)-mCherry (CHRM3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   EF1a

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSP1D1

    Plasmid

    #20061

    Purpose

    Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    deletion mutant (1-11) of MSP1; His-tag on N-term…

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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