We narrowed to 313 results for: Ky

1. ERK1C82C202

   Plasmid

   #164650

   Purpose

   Bacterial expression plasmid for His6-ERK1C82C202

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   ERK1C82C202 (MAPK1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   K71R/C144S/C178S/C183S/T202C/C271S

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSpCas9-hGeminin

   Plasmid

   #199344

   Purpose

   modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin

   Depositor

   Iain Hagan

   Article

   Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1

   Insert

   ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
   

   Use

   Tags

   cas9 c-term fused to hgemenin 1-110 fragment

   Expression

   Mammalian

   Mutation

   cas9 c-term fused to hgemenin 1-110 fragment

   Promoter

   cbh

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Δ1-60MEK1C222

   Plasmid

   #164641

   Purpose

   Bacterial expression plasmid for His6-ΔMEK1C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   S222C/C277S/C376S; residues 1-60 at N-terminus we…

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Δ1-60MEK1C218

   Plasmid

   #164643

   Purpose

   Bacterial expression plasmid for His6-ΔMEK1C218

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   S218C/C277S/C376S; residues 1-60 at N-terminus we…

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Δ1-60MEK1C218C222

   Plasmid

   #164645

   Purpose

   Bacterial expression plasmid for His6-ΔMEK1C218C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   S218C/S222C/C277S/C376S; residues 1-60 at N-termi…

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV2/9 REP-AAP-ΔCAP

   Plasmid

   #205991

   Purpose

   Rep and AAP protein expression for AAV CREATE libraries

   Depositor

   Viviana Gradinaru

   Article

   Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

   Insert

   AAV9 Rep and AAP proteins for CREATE libraries
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-U6-racRNA-A

   Plasmid

   #200824

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS puro HA-hUSP27X

   Plasmid

   #225718

   Purpose

   Bacterial expression of USP27X with HA tag

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X (USP27X Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-U6-racRNA-B

   Plasmid

   #200825

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-U6-racRNA-C

    Plasmid

    #200826

    Purpose

    AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter

    Depositor

    Xiao Wang

    Article

    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

    Inserts

    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    

    Use

    AAV

    Tags

    C-Far, Flag, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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