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Plasmid#190807PurposeExpress APOE E2 allele from Ubiquitin C promoter, co-expressing mCherryDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE2PromoterUbi CAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APOE-E4-T2A-Puro
Plasmid#190810PurposeExpress APOE E4 allele from Ubiquitin C promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE4PromoterUbi CAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV floxed hMyoD-IRES-dsRed
Plasmid#66632Purposeco-expresses CRE excisable human MyoD and dsRedDepositorInserthuman MyoD IRES dsRed (MYOD1 Human)
UseCre/Lox and LentiviralTagsExpressionMutationPromoterhUbCAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1-BFP
Plasmid#120577PurposeLentiviral plasmid to co-express a guide RNA and EBFP.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APPwt-T2A-mCherry
Plasmid#190803PurposeExpress wild-type APP from Ubiquitin C promoter, co-expressing mCherryDepositorInsertAPP (APP Human)
UseLentiviralTagsExpressionMutationPromoterUbi CAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1-TagBFP2
Plasmid#124773PurposeLentiviral plasmid to co-express a guide RNA and TagBFP2DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE-T2-IRES-His6-Ubiquitin
Plasmid#107107PurposeDesigned to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.DepositorInserthuman ubiquitin UBB (UBB Human)
UseTags6xHisExpressionMammalianMutationPromoterTetAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJPF0117
Plasmid#130267PurposeLentiviral vector for the co-expression of cytoplasmic bPAC and nuclear tagBFPDepositorInsertbPAC
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterhPGKAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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6C1A (MBD2)
Plasmid#119958PurposeBacterial expression for structure determinationDepositorInsertMBD2 (MBD2 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
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FUGW-APPIce-T2A-mCherry
Plasmid#190806PurposeExpress Icelandic mutant APP from Ubiquitin C promoter, co-expressing mCherryDepositorInsertAPP (APP Human)
UseLentiviralTagsExpressionMutationIcelandic (A673T)PromoterUbi CAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11a-dH3-dH4
Plasmid#105502PurposeCo-expression of Drosophila histones H3 and H4DepositorInsertdH3-dH4
UseTagsExpressionMutationPromoterAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-APOE-E4-T2A-Puro
Plasmid#190813PurposeExpress APOE E4 allele from tet-inducible promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE4PromoterTet-OAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-APOE-E3-T2A-Puro
Plasmid#190812PurposeExpress APOE E3 allele from tet-inducible promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE3PromoterTet-OAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-APOE-E2-T2A-Puro
Plasmid#190811PurposeExpress APOE E2 allele from tet-inducible promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE2PromoterTet-OAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-BFP
Plasmid#179651PurposeBFP co-transfection markerDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSuccessor
Plasmid#228391Purposeintended to be co-transformed along with pDawn-Ag43 to generate successor strain (SS-GFP) for multipattern biofilm lithographyDepositorInsertpConst23100-sfGFP__pLambda-DHFR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDifferentiation
Plasmid#228393Purposeintended to be co-transformed along with pDawn-Ag43 to generate differentiation strain (DS-mRuby2) for multipattern biofilm lithographyDepositorInsertpLambda-luxR+LVAtag__pLux-mRuby2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA
Plasmid#191528PurposeVector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabainDepositorInsertSLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via hdr using ouabainTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSFV3
Plasmid#92072PurposeEmpty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.DepositorTypeEmpty backboneUseEmpty expression plasmid, for run-off transcripti…TagsExpressionMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP
Plasmid#21373Purposeself-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APPSwe-T2A-Hygro
Plasmid#190805PurposeExpress Swedish mutant APP from Ubiquitin C promoter, co-expressing Hygro resistance geneDepositorInsertAPP (APP Human)
UseLentiviralTagsExpressionMutationSwedish (KM670/671NL)PromoterUbi CAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_R166QTS
Plasmid#191298PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1-CyOFP1
Plasmid#124771Purpose3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJRK248
Plasmid#173755Purposeco-injection plasmidDepositorInsertPrps-0::HygR::unc-54 3'UTR; Psqt-1::sqt-1::sqt-1 3' UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pESC-MET
Plasmid#222482PurposeCo-expression of two genes in yeast with FLAG and MYC tags and MET15 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-LYS
Plasmid#222483PurposeCo-expression of two genes in yeast with FLAG and MYC tags and LYS2 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-TYR
Plasmid#222484PurposeCo-expression of two genes in yeast with FLAG and MYC tags and TYR1 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-THR
Plasmid#222479PurposeCo-expression of two genes in yeast with FLAG and MYC tags and THR1 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-ADE
Plasmid#222480PurposeCo-expression of two genes in yeast with FLAG and MYC tags and ADE2 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-ARG
Plasmid#222481PurposeCo-expression of two genes in yeast with FLAG and MYC tags and ARG1 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBI/Rabaptin-5
Plasmid#171851Purposeinducible expression of Rabaptin-5 in mammalian cells via co-transfection with pTet-Off.DepositorInsertRabaptin-5 (human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH_35S/mEGFP
Plasmid#135321PurposemEGFP expression regulated by the CaMV35S promoter, co-expressed hygromycin phosphotransferase selectable marker (hpt)DepositorInsertmEGFP
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1-mOrange
Plasmid#124772Purpose3rd generation lentiviral plasmid to co-express a guide RNA and mOrangeDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ108 - pENTR221 - [cbr-unc-119(+)]
Plasmid#200367Purposecbr-unc-119 rescue gene for use as co-injection marker.DepositorInsert[cbr-unc-119(+)]
UseTagsExpressionWormMutationPromoterAvailable sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACE2 g1 + Cas9
Plasmid#153011PurposeA guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2DepositorInsertCas9 + ACE2 gRNA (ACE2 Human)
UseCRISPR and LentiviralTagsTagBFP2ExpressionMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJYDN2
Plasmid#162454PurposeYeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsDnbALFA nanobody and Ha and mycExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
ACE2 g4 + Cas9
Plasmid#153014PurposeA guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2DepositorInsertCas9 + ACE2 gRNA (ACE2 Human)
UseLentiviralTagsTagBFP2ExpressionMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJYDN2p
Plasmid#162455PurposePositive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).DepositorInsertAga2p-DnbALFA (AGA2 )
UseShuttle vector bacteria/yeastTagsDnbALFA nanobody and HA and mycExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only