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We narrowed to 5,049 results for: golden gate

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Showing: 1401 - 1450 of 5049 results

  1. U6m

    Plasmid
    #121019
    Purpose
    MoClo golden gate assembly BC part for BCD20 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD20
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121019-plasmid-map-sequence-id-238082

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  2. U7m

    Plasmid
    #121020
    Purpose
    MoClo golden gate assembly BC part for BCD16 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD16
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121020-plasmid-map-sequence-id-236180

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  3. U8m

    Plasmid
    #121021
    Purpose
    MoClo golden gate assembly BC part for BCD24 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD24
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121021-plasmid-map-sequence-id-236184

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  4. C84m

    Plasmid
    #120995
    Purpose
    MoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma 28
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120995-plasmid-map-sequence-id-236105

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  5. C95m

    Plasmid
    #121001
    Purpose
    MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7 RNA polymerase (Andy please check!)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121001-plasmid-map-sequence-id-236130

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  6. C63m

    Plasmid
    #120979
    Purpose
    MoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    lacI
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120979-plasmid-map-sequence-id-236033

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  7. C3m

    Plasmid
    #120956
    Purpose
    MoClo golden gate assembly CD part for sfGFP (Superfolder green fluorescent protein from DOI: 10.1038/nbt1172; slight variant on https://www.fpbase.org/protein/superfolder-gfp/)
    Depositor
    Richard Murray
    Insert
    sfGFP
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120956-plasmid-map-sequence-id-235970

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  8. P30m

    Plasmid
    #120942
    Purpose
    MoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pL-lacO1
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120942-plasmid-map-sequence-id-238038

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  9. C33m

    Plasmid
    #120968
    Purpose
    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AiiA degradase (Bennett, no ssra tag)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120968-plasmid-map-sequence-id-238044

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  10. C79m

    Plasmid
    #120991
    Purpose
    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdA antitoxin
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120991-plasmid-map-sequence-id-236074

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  11. V37m

    Plasmid
    #121043
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-mScar (ColE1/kan resistance destination vector with constitutive mScarlet expressing for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and mScarlet selection
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121043-plasmid-map-sequence-id-236355

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  12. C83m

    Plasmid
    #120994
    Purpose
    MoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pir protein required for R6K ori
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120994-plasmid-map-sequence-id-236103

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  13. C24m

    Plasmid
    #120964
    Purpose
    MoClo golden gate assembly CD part for lambda cI (repressor of pL or pOR1-OR2; use C64m for temperature-sensitive version). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    lambda-cI
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120964-plasmid-map-sequence-id-236100

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  14. C27m

    Plasmid
    #120965
    Purpose
    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    tetR (no deg tags)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120965-plasmid-map-sequence-id-236387

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  15. C98m

    Plasmid
    #121002
    Purpose
    MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Gp2 toxin
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121002-plasmid-map-sequence-id-236135

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  16. C51m

    Plasmid
    #120975
    Purpose
    MoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfYFP (has BbsI site)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120975-plasmid-map-sequence-id-236029

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  17. C115m

    Plasmid
    #121014
    Purpose
    MoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    TP901 integrase
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121014-plasmid-map-sequence-id-236321

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  18. P57m

    Plasmid
    #120949
    Purpose
    MoClo golden gate assembly AB part for pCin* (Adam Meyer's improved pCin promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pCin Adam Meyer
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120949-plasmid-map-sequence-id-235961

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  19. (P1)PA1lacO1

    Plasmid
    #251365
    Purpose
    IPTG-inducible Plac promoter variant with lacIq repressor and RiboJ
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    Promoter A1lacO-1
    Expression
    Bacterial
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    251365-plasmid-map-sequence-id-503679

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  20. (P6)RBSS3

    Plasmid
    #251388
    Purpose
    RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S3
    Expression
    Bacterial
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    251388-plasmid-map-sequence-id-503708

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  21. (P4)RBSS1

    Plasmid
    #251376
    Purpose
    Synthetic RBS based on Synechocystis ribosome 30S anti-SD
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S1
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251376-plasmid-map-sequence-id-503738

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  22. (P6)RBSS1

    Plasmid
    #251386
    Purpose
    Synthetic RBS based on Synechocystis ribosome 30S anti-SD
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S1
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251386-plasmid-map-sequence-id-503734

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  23. (P2)RBSS1

    Plasmid
    #251366
    Purpose
    Synthetic RBS based on Synechocystis ribosome 30S anti-SD
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S1
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251366-plasmid-map-sequence-id-503714

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  24. (P2)RBSS3

    Plasmid
    #251368
    Purpose
    RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S3
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251368-plasmid-map-sequence-id-503703

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  25. (P4)RBSS3

    Plasmid
    #251378
    Purpose
    RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S3
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251378-plasmid-map-sequence-id-503698

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  26. (P4)RBSS4

    Plasmid
    #251379
    Purpose
    RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S4
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251379-plasmid-map-sequence-id-503681

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  27. (P6)RBSS4

    Plasmid
    #251389
    Purpose
    RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S4
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251389-plasmid-map-sequence-id-503678

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  28. (P2)RBSS4

    Plasmid
    #251369
    Purpose
    RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S4
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251369-plasmid-map-sequence-id-503676

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  29. pNDMS239

    Plasmid
    #248096
    Purpose
    mScarlet-I fluorescent reporter for synthetic Mesorhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    mScarlet-I
    Expression
    Bacterial
    Promoter
    Meso_PsnifH
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248096-plasmid-map-sequence-id-495375

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  30. pKS1604

    Plasmid
    #239528
    Purpose
    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    239528-plasmid-map-sequence-id-487894

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  31. pKS1606

    Plasmid
    #239529
    Purpose
    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    239529-plasmid-map-sequence-id-487896

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  32. pN4

    Plasmid
    #233521
    Purpose
    Nb Vector Plasmid (under galactose induction), AmpR (E. coli) and TRP1 (yeast), Cen6/ARS
    Depositor
    Carl Denard
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Promoter
    pGAL1-10
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233521-plasmid-map-sequence-id-471179

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  33. pKS1620

    Plasmid
    #239551
    Purpose
    Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrCPR-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239551-plasmid-map-sequence-id-478368

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  34. pKS1617

    Plasmid
    #239548
    Purpose
    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239548-plasmid-map-sequence-id-478367

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  35. pKS1618

    Plasmid
    #239549
    Purpose
    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239549-plasmid-map-sequence-id-478366

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  36. pKS1334

    Plasmid
    #239508
    Purpose
    Express CDT2 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    NcCDT2-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    239508-plasmid-map-sequence-id-477192

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  37. pKS1619

    Plasmid
    #239550
    Purpose
    Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrCPR-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239550-plasmid-map-sequence-id-475997

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  38. pKS1601

    Plasmid
    #239526
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239526-plasmid-map-sequence-id-475905

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  39. pKS1531

    Plasmid
    #239521
    Purpose
    Express truncated VoGES and VvGT14 in the peroxisome for synthesis of geranyl _-glucoside; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    tVoGES-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239521-plasmid-map-sequence-id-475896

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  40. pVL1337

    Plasmid
    #239541
    Purpose
    Express Yea4p membrane protein cargo fused directly to Pex15 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-ScPex15(315-383)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239541-plasmid-map-sequence-id-475879

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  41. pKS1574

    Plasmid
    #239522
    Purpose
    Express Yea4p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-ScYea4p-mTurquoise2
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239522-plasmid-map-sequence-id-475877

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  42. pVL1280

    Plasmid
    #239539
    Purpose
    Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    NcCDT1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239539-plasmid-map-sequence-id-475864

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  43. pAIP4_3W4Q

    Plasmid
    #234236
    Purpose
    Heterologous protein expression of beta-lactamase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3W4Q
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234236-plasmid-map-sequence-id-464200

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  44. pAIP4_3ILS

    Plasmid
    #234215
    Purpose
    Heterologous protein expression of norsolorinic acid synthase (NSAS) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3ILS
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234215-plasmid-map-sequence-id-464180

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  45. pAIP4_5NJO

    Plasmid
    #234202
    Purpose
    Heterologous protein expression of putative cupin_2 domain-containing isomerase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5NJO
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234202-plasmid-map-sequence-id-464167

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  46. pAIP4_1ZHV

    Plasmid
    #234197
    Purpose
    Heterologous protein expression of aspartate kinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1ZHV
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234197-plasmid-map-sequence-id-464162

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  47. pAIP4_1NG6

    Plasmid
    #234191
    Purpose
    Heterologous protein expression of YqeY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1NG6 (yqeY Bacillus subtilis)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234191-plasmid-map-sequence-id-464156

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  48. pAIP4_4QB4

    Plasmid
    #234187
    Purpose
    Heterologous protein expression of thermonuclease (TNase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4QB4
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234187-plasmid-map-sequence-id-464152

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  49. pAIP4_2OHW

    Plasmid
    #234178
    Purpose
    Heterologous protein expression of YueI in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2OHW (yueI Bacillus subtilis)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234178-plasmid-map-sequence-id-464143

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  50. pAIP4_4CFI

    Plasmid
    #234163
    Purpose
    Heterologous protein expression of flagellin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4CFI (QUF21_RS17235 Burkholderia pseudomallei K96243)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234163-plasmid-map-sequence-id-464129

    Enlarge

Showing: 1401 - 1450 of 5049 results