We narrowed to 581 results for: Nut

1. pCMV_SEPT9_i3-14-GFP11

   Plasmid

   #180346

   Purpose

   mammalian expression of human SEPT9_i3 fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN9_v3 (SEPTIN9 Human)
   

   Use

   Tags

   GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV2_hSyn_Aurora_CA_Citrine

   Plasmid

   #98219

   Purpose

   Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Aurora_C128A gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

   Promoter

   human synapsin

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6

   Plasmid

   #180313

   Purpose

   bacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Use

   Tags

   His6-TEV and sfCherry2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_SEPT2 NCmut-msfGFP

   Plasmid

   #180320

   Purpose

   mammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN2 (SEPTIN2 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT2 F20D, V27D

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_SEPT9_i3 Gmut-msfGFP

   Plasmid

   #180326

   Purpose

   mammalian expression of human SEPT9_i3 Gmut fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN9_v3 (SEPTIN9 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i3 W502A, H512D

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP

   Plasmid

   #180311

   Purpose

   bacterial expression of human SEPT2 NCmut fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN2 (SEPTIN2 Human)
   

   Use

   Tags

   His6-TEV and msfGFP

   Expression

   Bacterial

   Mutation

   SEPT2 F20D, V27D

   Promoter

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV_SEPT9_i3-mApple-CAAX

   Plasmid

   #180324

   Purpose

   mammalian expression of human SEPT9_i3 fused to monomeric Apple and CAAX (HRas)

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN9_v3 (SEPTIN9 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV_SEPT9_i3 NCmut-msfGFP

   Plasmid

   #180325

   Purpose

   mammalian expression of human SEPT9_i3 NCmut fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN9_v3 (SEPTIN9 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i3 I263D, M270D

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i1-14-GFP10

   Plasmid

   #180348

   Purpose

   mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN9 (SEPTIN9 Human)
   IRES-TagBFP
   

   Use

   Lentiviral

   Tags

   GFP10 and GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   bidirectional TRE

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i3-14-GFP10

    Plasmid

    #180349

    Purpose

    mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    bidirectional TRE

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRIP TRE BI_SEPT9_i1-14-GFP10_GFP11-14-SEPT7 Gmut2

    Plasmid

    #180359

    Purpose

    mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    SEPT7 H279D

    Promoter

    bidirectional TRE

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2

    Plasmid

    #180361

    Purpose

    mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    SEPT7 H279D

    Promoter

    bidirectional TRE

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP_SEPT6

    Plasmid

    #180312

    Purpose

    bacterial co-expression of human SEPT2 NCmut fused to monomeric superfolder GFP and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    SEPT2 F20D, V27D

    Promoter

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-TAC-OSKM

    Plasmid

    #80481

    Purpose

    piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB-TAC-MKOS

    Plasmid

    #80484

    Purpose

    piggyBac transposon for dox-inducible expression of the MKOS polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    MKOS (Myc, Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i1 Gmut-14-GFP10

    Plasmid

    #180355

    Purpose

    mammalian co-expression of human SEPT9_i1 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 )
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    SEPT7 W269A, H279D and SEPT9_i1 W520A, H530D

    Promoter

    bidirectional TRE

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i3 Gmut-14-GFP10

    Plasmid

    #180356

    Purpose

    mammalian co-expression of human SEPT9_i3 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 )
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    SEPT7 W269A, H279D and SEPT9_i3 W502A, H512D

    Promoter

    bidirectional TRE

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-TAC-OKMS

    Plasmid

    #80480

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OKMS (Pou5f1, Klf4, Myc, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-TAC-OK+9MS

    Plasmid

    #80482

    Purpose

    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OK+9MS (Pou5f1, Klf4, Myc, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [1-483]

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-TAC-ERP2-(N-HA)KRAS_G12D

    Plasmid

    #201254

    Purpose

    Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

    Depositor

    Alexander Kleger

    Article

    Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

    Insert

    KRAS (KRAS Human)
    

    Use

    Piggybac transposon

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    changed Gly (G) 12 to Asp (D)

    Promoter

    TRE

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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