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Human Coxsackievirus A16 strain G10 2A protease

Plasmid

#228632

Purpose

Bacterial expression of the Coxsackievirus A16 (G-10) 2A protease this construct can be used for biochemical assay and to obtain crystals in the space group C1 2 1(PDB 8POA)

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus Coxsackievirus A16 2A protease (HEVAgp1 Coxsackievirus 16)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pRSF-KpDcr1deltaNdeltaC

Plasmid

#32030

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(107-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1delta2d(E224Q)

Plasmid

#32029

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-260)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

E224Q

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1delta2d

Plasmid

#32028

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-260)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(K217A)

Plasmid

#32026

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

K217A

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(N184A)

Plasmid

#32025

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

N184A

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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Flag-SENP1

Plasmid

#17357

Depositor

Edward Yeh

Article

Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

Insert

SENP1 (SENP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 19, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-SENP1m

Plasmid

#17358

Depositor

Edward Yeh

Article

Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

Insert

SENP1 (SENP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

R630L, K631M

Promoter

Available since

March 19, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2 SENP2

Plasmid

#13382

Depositor

Mary Dasso

Article

Hang et al J Biol Chem. 2002 May 31. 277(22):19961-

Insert

SENP2 (SENP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

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p361_DG

Plasmid

#234683

Purpose

Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLJSRSF7

Plasmid

#64693

Purpose

His-MBP-Smt3-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Use

Tags

CBD, HisTag, MBP, NpuDnaE-N102, and Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pTXG

Plasmid

#52280

Purpose

Bacterial expression of TARGET proteins C-terminally fused to GyrA intein and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Type

Empty backbone

Use

Tags

GyrA-intein-GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCARSF63

Plasmid

#64695

Purpose

H6-TRX-Smt3-GB1-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Use

Tags

CBD, HisTag, NpuDnaE-N102, Smt3, and TRX

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLJSRSF3

Plasmid

#64692

Purpose

His-GST-Smt3-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Use

Tags

CBD, GST, HisTag, NpuDnaE-N102, and Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCARSF85

Plasmid

#64694

Purpose

H6-TF-Smt3-GB1-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Use

Tags

CBD, HisTag, NpuDnaE-N102, Smt3, and TF

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(GiVL-1)

Plasmid

#32032

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

H123-N143 replaced by P642-Y645 of GiDicer

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1(1-384)

Plasmid

#32031

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(1-384)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(GiVL-2)

Plasmid

#32034

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

N195-M215 replaced by P697-D714 of GiDicer

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(E147Q/D151N)

Plasmid

#32024

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

E147Q/D151N

Promoter

T7

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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10xHis-ataxin-3 *SIM-HA

Plasmid

#89983

Purpose

Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

10xHis and HA

Expression

Mammalian

Mutation

mutated aa 162IFVV to 162AFAA

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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