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Mito-Lapronic/pGW1

Plasmid

#140754

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Mito-Lapronic

Tags

Mitochondrial Sequence Signal

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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Cry2(1-531)-mCh-BAXS184E

Plasmid

#117238

Purpose

Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

Cry2531-mCh-BAXS184E

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pENO1-iRFP-NATr

Plasmid

#129731

Purpose

Integration plasmid to overexpress iRFP in Candida albicans

Depositor

Robert Wheeler

Article

Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

Insert

iRFP-Candida albicans codon-optimized

Expression

Yeast

Promoter

ENO1 from Candida albicans

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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BRAC/pcDNA3

Plasmid

#51967

Purpose

mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

Depositor

Takeharu Nagai

Article

Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

Insert

BRAC

Expression

Mammalian

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
Tom20-CIB-stop

Plasmid

#117243

Purpose

Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

CIBN

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET/LEGH

Plasmid

#108226

Purpose

Bacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag

Depositor

Didier Picard

Article

Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

Insert

LBT-EGFP-His6

Expression

Bacterial

Promoter

T7, lacO

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSopBC460S

Plasmid

#112514

Purpose

PsopB driven sopB (catalytically inactive) and sigE

Depositor

Olivia Steele-Mortimer

Article

Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

Insert

sopB (catalytically inactive)/sigE operon

Expression

Bacterial

Mutation

SopB C460S

Promoter

PsopB

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET11a-TAX-L10-STEPtag

Plasmid

#159375

Purpose

Expresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

TAX-L10-STEPtag

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET11a-STEPtag-L10-TAX

Plasmid

#159374

Purpose

Expresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

STEPtag-L10-TAX

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pST96/LBT-MCS

Plasmid

#108227

Purpose

Bacterial expression expression of protein with N-terminal lanthanide binding tag

Depositor

Didier Picard

Article

Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

Insert

LBT

Expression

Bacterial

Promoter

T7, lacO

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pADH118-17

Plasmid

#91727

Purpose

NAT-marked C. albicans URA3-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

NAT 2of2, pSNR52, C. albicans URA3-specific gRNA, LEU2 2of2

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

Plasmid

#217478

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1s

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

Plasmid

#217479

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1b

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET16b-RPA4/His14 (MSW#949)

Plasmid

#208075

Purpose

Expression of human Replication Protein A 4 and His-tagged RPA3 in E.coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA4, RPA14(His-tagged)

Tags

10xHis tag on RPA14

Expression

Bacterial

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET3a-FruK

Plasmid

#186256

Purpose

Overexpression of FruK for purification

Depositor

Liskin Swint-Kruse, Emile Van Schaftingen

Article

Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

Insert

1-phosphofructokinase (fruK E. coli)

Expression

Bacterial

Promoter

T7

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pKRaION1-N1

Plasmid

#177801

Purpose

Mammalian expression of green fluorescent potassium indicator KRaION1

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

KRaION1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pKRaION2-N1

Plasmid

#177802

Purpose

Mammalian expression of green fluorescent potassium indicator KRaION2

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

KRaION2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Laconic/pcDNA3.1(-)

Plasmid

#44238

Purpose

a genetically-encoded sensor for lactate concentration, transport and metabolic flux

Depositor

Luis Felipe Barros

Article

San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

Insert

Laconic (LACtate Optical Nano Indicator from CECs)

Use

Lactate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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pETDuet-LahT150

Plasmid

#166785

Purpose

Cleavage of double glycine motif in peptides to remove the leader sequence

Depositor

Wilfred van der Donk

Article

Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

Insert

LahT150

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Setd2CD

Plasmid

#235579

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Setd2 (Setd2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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