Addgene

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pAAV.hSynapsin.TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

Plasmid

#187101

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-SF-iGluSnFR

Use

AAV

Tags

TagBFP-P2A

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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IDP432

Plasmid

#87000

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

SNAP-Nlg1AB-1xλN(i)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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IDP431

Plasmid

#86999

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

CLIP-Nrx1B-4xλN(i)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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IDP455

Plasmid

#87001

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

barcode landing site Gfp-4xboxB

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pIM63

Plasmid

#193545

Purpose

pENTRY clone for NLR Integrated Domain ID#19

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#19

Use

Pentry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pIM65

Plasmid

#193547

Purpose

pENTRY clone for NLR Integrated Domain ID#14

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#14

Use

Pentry clone

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pIM66

Plasmid

#193548

Purpose

pENTRY clone for NLR Integrated Domain ID#12

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#12

Use

Pentry clone

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pIM60

Plasmid

#193542

Purpose

pENTRY clone for NLR Integrated Domain ID#22

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#22

Use

Pentry clone

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pIM45

Plasmid

#193539

Purpose

pENTRY clone for NLR Integrated Domain ID#39

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#39

Use

Pentry clone

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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IDP254

Plasmid

#87004

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

postCLAP

Use

Synthetic Biology

Tags

spGFP (1-10)

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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IDP253

Plasmid

#87003

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

preCLAP

Use

Synthetic Biology

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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IDP379

Plasmid

#87006

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

postLib-mRFP-4xboxB

Use

Synthetic Biology

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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IDP378

Plasmid

#87005

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

preLib-bfp-4xboxB

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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TRIPZ-CTID195-GSQ-SUMO1nc-PURO

Plasmid

#208035

Purpose

Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

Depositor

James Sutherland, Rosa Barrio

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

SUMO1nc (SUMO1 Human)

Use

Lentiviral

Tags

Myc, C-terminal Split-TurboID

Expression

Mutation

Q94P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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TRIPZ-CTID195-GSQ-UBnc-PURO

Plasmid

#208037

Purpose

Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

Depositor

James Sutherland, Rosa Barrio

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

Ubnc (UBC Human)

Use

Lentiviral

Tags

Myc, C-terminal Split-TurboID

Expression

Mutation

L73P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNTI819

Plasmid

#216628

Purpose

Cal-ID (V5) for mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-StrepHA-SUMO2

Plasmid

#66867

Purpose

Mammalian expression of SUMO2 with a strep and an HA tag

Depositor

Ying Liu

Article

Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

Insert

SUMO2 (SUMO2 Human)

Use

Tags

HA and Strep

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3

Plasmid

#25748

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pEN_TTmcs

Plasmid

#25755

Purpose

Entry vector with TRE-tight promoter followed by multiple cloning site.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pEN_Tmcs

Plasmid

#25751

Purpose

Entry vector with TRE promoter followed by multiple cloning site.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge