We narrowed to 845 results for: nanobody

1. pTubulin-miRFP670nano3

   Plasmid

   #184666

   Purpose

   Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3-human alpha-tubulin
   

   Use

   Tags

   miRFP670nano3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQCXIP-cathepsinD-YMH

   Plasmid

   #182644

   Purpose

   Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   cathepsinD
   

   Use

   Retroviral

   Tags

   His6-tag, TS site from proCCK, and myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSb_init containing the MBP sybody Sb_MBP#1

   Plasmid

   #132699

   Purpose

   expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   sybody_MBP#1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. NbV5-HALOTAG

   Plasmid

   #201482

   Purpose

   Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-HALOTAG
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDV5 containing Sb-loop

   Plasmid

   #132695

   Purpose

   pRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   Sb_loop including 5' and 3' flanking sequences used for ribosome display
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. NbV5-mCherry

   Plasmid

   #201474

   Purpose

   Indirect microscopy/imaging of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LgBit-NbALFA

   Plasmid

   #201484

   Purpose

   NanoBit detection of ALFA-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   LgBit-NbALFA
   

   Use

   Tags

   LgBit

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTP341 pHAGE2 CMV TagBFP-3xFLAG

   Plasmid

   #199391

   Purpose

   Lentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cells

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   TagBFP-3xFLAG
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET51b(+)_ALFA-tag-SNAP

   Plasmid

   #136628

   Purpose

   Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-NbALFA
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIR-FbGFPY58/C-PKI

    Plasmid

    #184685

    Purpose

    NIR-Fb-based PKA inhibitor

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbGFPY58/C-PKI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.0_nucLAMA-G97 IRES EGFP

    Plasmid

    #136620

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS (IRES EGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. NbAlfa-LgBit

    Plasmid

    #201483

    Purpose

    NanoBit detection of ALFA-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbALFA-LgBit
    

    Use

    Tags

    LgBit

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

    Plasmid

    #199346

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCOLA-Spec-yibDp-Erv1p

    Plasmid

    #212094

    Purpose

    Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    Sulfydryl oxidase (ERV1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Gal4DBD-GBP2

    Plasmid

    #49439

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP2-Gal4 DNA binding domain fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. NbV5-HiBiT

    Plasmid

    #201489

    Purpose

    NanoBit detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbV5-HiBiT
    

    Use

    Tags

    HiBit

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBMN-dGBP1-TagBFP

    Plasmid

    #80087

    Purpose

    MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Insert

    dGBP1-TagBFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTP924 pHAGE2 CMV GFP

    Plasmid

    #199392

    Purpose

    Lentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2+_Flag-myc-NES-Tir1

    Plasmid

    #117717

    Purpose

    mRNA synthesis of Flag-myc-NES-TIR1 for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    TRANSPORT INHIBITOR RESPONSE 1
    

    Use

    Tags

    Flag-Myc-NES

    Expression

    Mammalian

    Mutation

    codon optimized TIR1 for murine expression

    Promoter

    SP6

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRDV5 containing Sb-convex

    Plasmid

    #132696

    Purpose

    pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Sb_convex including 5' and 3' flanking sequences used for ribosome display
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge