We narrowed to 4,553 results for: pcr

1. pCRII-Sema4d-ISH-2kb

   Plasmid

   #68032

   Purpose

   Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

   Depositor

   Roland Friedel

   Article

   Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

   Insert

   Semaphorin-4D (Sema4d Mouse)
   

   Use

   Mrna in situ hybridization probe

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRII-Plxnb1-ISH-2kb

   Plasmid

   #68035

   Purpose

   Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

   Depositor

   Roland Friedel

   Article

   Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

   Insert

   Plexin-B1 (Plxnb1 Mouse)
   

   Use

   Mrna in situ hybridization probe

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS596 tet-hCMV-GFPcre

   Plasmid

   #11961

   Depositor

   Brian Sauer

   Article

   Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

   Insert

   GFP-cre (cre bacteriophage P1)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 28, 2006

   Availability

   Academic Institutions and Nonprofits only

4. (G38) pCRII-Wnt10b/b-globin

   Plasmid

   #25877

   Depositor

   Ormond MacDougald

   Article

   Longo et al J Biol Chem. 2004 Aug 20. 279(34):35503-

   Insert

   Wnt10b / b-globin (Wnt10b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pCRII-Plxnb3-ISH-2kb

   Plasmid

   #68037

   Purpose

   Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

   Depositor

   Roland Friedel

   Article

   Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

   Insert

   Plexin-B3 (Plxnb3 Mouse)
   

   Use

   Mrna in situ hybridization probe

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRII-Sema4g-ISH-2kb

   Plasmid

   #68034

   Purpose

   Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

   Depositor

   Roland Friedel

   Article

   Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

   Insert

   Semaphorin-4G (Sema4g Mouse)
   

   Use

   Mrna in situ hybridization probe

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXTK011 [Marker_SpcR-StrR-aadA_PartType4]

   Plasmid

   #229248

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_SpcR-StrR-aadA_PartType4

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   SpcR-StrR-aadA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXTK004 [Marker_SpcR-StrR-aadA_PartType2]

   Plasmid

   #229241

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_SpcR-StrR-aadA_PartType2

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   SpcR-StrR-aadA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPEY-GAL-Hyg-ADE2

   Plasmid

   #232104

   Purpose

   Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   GAL7

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRII-mogat3b in situ probe

    Plasmid

    #232136

    Purpose

    zebrafish mogat3b anti-sense in situ probe, linearize with BamHI, T7 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    mogat3b
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcRII-mogat3a in situ probe

    Plasmid

    #232135

    Purpose

    zebrafish mogat3a anti-sense in situ probe, linearize with NotI, SP6 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    mogat3a
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRII-dgat2 in situ probe

    Plasmid

    #232134

    Purpose

    zebrafish dgat2 anti-sense in situ probe, linearize with BamHI, T7 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    dgat2 (dgat2 Zebrafish)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7

    Plasmid

    #196846

    Purpose

    backbone for gRNA cloning of dpspCas13b tagged by 2xPP7

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b

    Plasmid

    #196847

    Purpose

    backbone for gRNA cloning of dpspCas13b tagged by 1xMS2

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCR 2.1-TOPO Fzd6HA

    Plasmid

    #208363

    Purpose

    Extracellular HA-tagged Fzd6 (mouse)

    Depositor

    Maureen Cetera, Danelle Devenport

    Article

    Cetera et al Development. 2023 Aug 25:dev.202078. doi

    Insert

    Fzd6 (Fzd6 Mouse)
    

    Use

    Cloning vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR-U6-gRNA-TRE3G-Nluc

    Plasmid

    #192659

    Purpose

    A plasmid encoding TRE3G sgRNA and TRE3G-promoter-Nluc

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Nluc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, TRE3G

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR-U6-gRNA-miniCMV-Nluc

    Plasmid

    #192657

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-Nluc

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Nluc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, miniCMV

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPaint-mNeon-F-gB-PuroR [M1G]

    Plasmid

    #171803

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-gB-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPaint-mScarlet-F-gB-BlastR [M1G]

    Plasmid

    #171816

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-gB-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPaint-mScarlet-F-gB-PuroR [M1G]

    Plasmid

    #171812

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-gB-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge