We narrowed to 1,516 results for: pet-28a

1. pET28a-anticalin (S87amb)-His

   Plasmid

   #168044

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Ser 87 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-anticalin (N116amb)-His

   Plasmid

   #168045

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Asn 116 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-anticalin (N21amb)-His

   Plasmid

   #168041

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Asn 21 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-anticalin (I55amb)-His

   Plasmid

   #168042

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Ile 55 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_6HT-TBP6.7(delta C-term)

   Plasmid

   #112731

   Purpose

   This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(delta C-term)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   delta C-term

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-Hook3 D102A, E108A

   Plasmid

   #74618

   Purpose

   Expresses a double mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   D102A, E108A

   Promoter

   T7

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pet28a His6-HSPB7 (zebrafish) WT

   Plasmid

   #108917

   Purpose

   Express WT Zebrafish His6-HSPB7 in bacteria

   Depositor

   Yimon Aye

   Article

   Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

   Insert

   His6-HSPB7 (hspb7 Zebrafish)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-SdrG_N2N3-B1-B2-HIS-ybbr

   Plasmid

   #117979

   Purpose

   SdrG N2 and N3, B1 and B2 domains with C-terminal ybbr tag for covalent immobilization

   Depositor

   Hermann Gaub

   Article

   Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

   Insert

   SdrG_N2N3-B1-B2
   

   Use

   Tags

   H3V 3C, HIS, and ybbr

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-SdrG_N2N3-B1-HIS-ybbr

   Plasmid

   #117980

   Purpose

   SdrG N2 and N3, B1 domains with C-terminal ybbr tag for covalent immobilization

   Depositor

   Hermann Gaub

   Article

   Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

   Insert

   SdrG_N2N3-B1
   

   Use

   Tags

   H3V 3C, HIS, and ybbr

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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10. PET-28a-YTH domain of YTHDC2

    Plasmid

    #228779

    Purpose

    Protein expression plasmid for YTH domain of YTHDC2 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDC2 (YTHDC2 Human)
    

    Use

    Tags

    6*His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a(+)_beta2M (MHC Class I light)

    Plasmid

    #187357

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    beta2M (MHC Class I light)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-28a-mCherry-MED1-IDR

    Plasmid

    #194545

    Purpose

    Bacterial Expression of 6xHis mCherry-MED1-IDR Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    MED1 (MED1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pet28a-His6-Halo-TEV-Nrf2

    Plasmid

    #62455

    Purpose

    Halo-His6 tagged Nrf2 for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Nrf2 (NFE2L2 Human)
    

    Use

    Tags

    His6-Halo-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. PET-28a-YTH domain of YTHDF2

    Plasmid

    #228776

    Purpose

    Protein expression plasmid for YTH domain of YTHDF2 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    6*His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-28a(+)-T7-OmpA-MNaseA-6xHis

    Plasmid

    #214808

    Purpose

    Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

    Depositor

    Peter Sarin

    Article

    Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

    Insert

    OmpA-Micrococcal nuclease A
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-28a+GFP-CAMSAP3 CKK

    Plasmid

    #59033

    Purpose

    expresses CKK domain of CAMSAP3 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP3 CKK domain (CAMSAP3 Mouse)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. PET-28a-YTH domain of YTHDF1

    Plasmid

    #228775

    Purpose

    Protein expression plasmid for YTH domain of YTHDF1 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDF1 (YTHDF1 Human)
    

    Use

    Tags

    6*His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-GGG-ELP(40nm)-Cys

    Plasmid

    #91571

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-ELP(40nm)-Cys
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PET-28a-YTH domain of YTHDF3

    Plasmid

    #228777

    Purpose

    Protein expression plasmid for YTH domain of YTHDF3 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDF3 (YTHDF3 Human)
    

    Use

    Tags

    6*His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-28a RUVBL2 Full Length

    Plasmid

    #159142

    Purpose

    Expresssion and protein purification of full length RUVBL2

    Depositor

    Eric Erquan Zhang

    Article

    Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

    Insert

    RUVBL2 (RUVBL2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge