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We narrowed to 705 results for: transfection

Showing: 561 - 580 of 705 results

  1. pGfa2(ABDx3)-nLac

    Plasmid
    #53133
    Purpose
    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cells
    Depositor
    Michael Brenner
    Article
    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    -2163 to +47, with 3 copies of 3 copies of the gf…
    Promoter
    human Gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53133-plasmid-map-sequence-id-189228

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  2. B2M Bulldozer (gRNA crB2M_13)

    Plasmid
    #84381
    Purpose
    This plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cells
    Depositor
    Chad Cowan
    Article
    Mandal et al Cell Stem Cell. 2014 Nov 6;15(5):643-52.
    Insert
    B2M Bulldozer crB2M_13 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 promoter
    Available since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84381-plasmid-map-sequence-id-189203

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  3. pCSCRET2(#323)

    Plasmid
    #184058
    Purpose
    cyclofen-inducible CRE activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1
    Insert
    6myc-CRE-ERT2
    Use
    Synthetic Biology
    Tags
    6xMyc
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184058-plasmid-map-sequence-id-365949

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  4. pAAV-syn-FLEX-axon-jYCaMP1s

    Plasmid
    #135421
    Purpose
    Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant
    Depositor
    Kaspar Podgorski
    Article
    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s
    Has Service
    AAV1
    Insert
    jYCaMP1s
    Use
    AAV
    Tags
    GAP43 axon targeting sequence
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Jan. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    135421-plasmid-map-sequence-id-268929

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  6. pGfa(B3)-nLac

    Plasmid
    #53132
    Purpose
    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional B sites, has about 75 times higher activity than pGfa2-nLac in transfected cells
    Depositor
    Michael Brenner
    Article
    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    -2163 to +47, with 3 copies of the gfa2 B segment…
    Promoter
    human Gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53132-plasmid-map-sequence-id-82721

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  9. pCSmEn2ERT(#117)

    Plasmid
    #184079
    Purpose
    cyclofen-inducible chicken EN2 (wt) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184079-plasmid-map-sequence-id-365956

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  10. pCSmEn2n4ERT(#234)

    Plasmid
    #184082
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2(n4)-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    chkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184082-plasmid-map-sequence-id-365718

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  11. pCSmEn2SRERT(#231)

    Plasmid
    #184080
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2(SR)-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184080-plasmid-map-sequence-id-365710

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  12. pCSmEn25EERT(#232)

    Plasmid
    #184081
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2(5E)-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …
    Promoter
    sCMV+SP6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184081-plasmid-map-sequence-id-363924

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  14. pMSCVpuro-Mito-Pericam

    Plasmid
    #87381
    Purpose
    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
    Depositor
    Björn Stork
    Article
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    Mito-Pericam [ratiometric]
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87381-plasmid-map-sequence-id-212242

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Showing: 561 - 580 of 705 results