We narrowed to 18,727 results for: Mut

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D-raf C295S

Plasmid

#39768

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

C295S

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf F290I

Plasmid

#39766

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

F290I

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf R217L+S620A

Plasmid

#39765

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

R217L and S620A

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf R217L+C295S

Plasmid

#39764

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

R217L and C295S

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/GS-DJ1-E18D-V5

Plasmid

#29346

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

V5 epitope

Expression

Mammalian

Mutation

E18D

Available Since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/GS-DJ1-E18N-V5

Plasmid

#29341

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

V5 epitope

Expression

Mammalian

Mutation

Glu18Asn

Available Since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pET21-MRPL13

Plasmid

#31344

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein L13 mature form (MRPL13 Human)

Tags

His

Expression

Bacterial

Mutation

Mature form after removal of the predicted mitoch…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pENTR4_EWSR1_C2G_R650G

Plasmid

#26371

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Entry vector

Mutation

R650G

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
UAS-u(L)Cas9

Plasmid

#127385

Purpose

Expresses Cas9 at low level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(L)-Cas9

Use

CRISPR

Expression

Insect

Availability

Academic Institutions and Nonprofits only

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pET-17b_K490clm_E215C

Plasmid

#169995

Purpose

Kinesin KIF5B K490 cysteine light mutant with E215C mutation for single cysteine labeling

Depositor

Ron Vale

Article

Niekamp et al Proc Natl Acad Sci U S A. 2019 Mar 5;116

Insert

kinesin family member 5B, isoform CRA_a

Tags

6xHIS

Expression

Bacterial

Mutation

C7S, C65A, C168A, C174S, E215C, C294A, C330S, C42…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG

Plasmid

#70183

Purpose

Inducible expression of guide RNA with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

H1-Tet-sgrna cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

Plasmid

#138610

Purpose

Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Inserts

Hyperactive AID
T7 RNA Polymerase
tdTomato

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD

Plasmid

#98669

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-his-BACH1 WT

Plasmid

#17642

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pRDA_355

Plasmid

#187159

Purpose

Lentiviral expression of doxycycline inducible customizable Sp Cas9 sgRNA

Depositor

John Doench, David Root

Article

Aquilanti et al Neuro Oncol. 2023 Jul 6;25(7):1275-1285.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

Plasmid

#171588

Purpose

Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

Bxb1 integrase
iCasp9
BFP
rtTA3G

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 deltaN89 Beta-catenin

Plasmid

#19288

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

N-terminal truncation, deleted aa 1-89

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
FUCas9Cherry

Plasmid

#70182

Purpose

Expresses Cas9 with fluorescent Cherry reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

humanised S.pyogenes cas9

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

hUbC

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgRNA

Plasmid

#124844

Purpose

Vector for Cre-dependent expression of SaCas9

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SaCas9, gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS and NLS-3xHA

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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