We narrowed to 13,291 results for: cDNA

1. pcDNA3-N-Flag-NLRP3 711-1034

   Plasmid

   #75141

   Purpose

   Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   fragment encoding amino acids 711-1034

   Promoter

   CMV

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEEF1D-FLAG (pcDNA 3.1+) LG6-1

   Plasmid

   #37365

   Depositor

   David Litchfield

   Article

   Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

   Insert

   EEF1D (EEF1D Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 17, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pCDNA3-mouse PKA-RIalpha-mEGFP

   Plasmid

   #45525

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA regulatory subunit RI alpha tagged by mEGFP
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3puro-mGFP-FMR1 isoform1-sh1Resistant

   Plasmid

   #225462

   Purpose

   Express mEGFP-fusion protein of FMR1 isoform 1

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FMR1 isoform 1 (FMR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG

   Plasmid

   #225245

   Purpose

   Mammalian expression of iPASnFR-H in the nucleus

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-H
   

   Use

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

   Plasmid

   #86827

   Purpose

   Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   FLAG-NLS-MS2-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 20, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008

   Plasmid

   #187918

   Purpose

   Expresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase basic protein 1
   

   Use

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   CMV Promoter

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST pcDNA5-FRT/TO-eGFP-Rif1

   Plasmid

   #52506

   Purpose

   mammalian expression vector of Rif1 siRNA resistant

   Depositor

   Daniel Durocher

   Article

   Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

   Insert

   RIF1 (RIF1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   siRNA resistant sequence: ATAGTAC

   Promoter

   Available Since

   June 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-WT

   Plasmid

   #218834

   Purpose

   Expresses N-terminal BirA*-tagged human wild type TLK1 variant 5 for BioID experiments.

   Depositor

   Travis Stracker

   Insert

   TLK1 (TLK1 Human)
   

   Use

   Tags

   FLAG-BirA*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 (+) -myc-OGA(GS-544)C181

    Plasmid

    #193996

    Purpose

    plasmid encoding myc-tagged OGA(GS-544)C181

    Depositor

    Christina Woo

    Article

    Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

    Insert

    myc-OGA(GS-544)C181
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-NLS-iPASnFR-L-mScarlet3-3xFLAG

    Plasmid

    #225246

    Purpose

    Mammalian expression of iPASnFR-L in the nucleus

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-FRT-TetO-U2B''-fSNAPc

    Plasmid

    #106988

    Purpose

    open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    UB2" (SNRPB2 Human)
    

    Use

    Tags

    fSNAP

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-LoxP-mKate-LoxP-EGFP

    Plasmid

    #167940

    Purpose

    CMV promoter-LoxP-mKate-LoxP-EGFP

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    LoxP-mKate-LoxP-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT-TetO-U1-70K-fSNAPc

    Plasmid

    #106987

    Purpose

    open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    U1-70K (SNRNP70 Human)
    

    Use

    Tags

    fSNAP

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO_Vim-SNAP-tag2

    Plasmid

    #226518

    Purpose

    Mamalian cell expression of intermediate filaments (vimentin) fused to SNAP-tag2

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    Vimentin-SNAP-tag2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

    Plasmid

    #171437

    Purpose

    Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    c-Fos-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-MUC2 D1 with cleavable His tag

    Plasmid

    #181813

    Purpose

    Expresses human MUC2 D1 with cleavable His tag, residues 21-389

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    Muc2 D1 (MUC2 Human)
    

    Use

    Tags

    6xHistadine followed by TEV cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

    Plasmid

    #158953

    Purpose

    Express human RAD51(T309A) mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    RAD51 (RAD51 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    changed threonine 309 to alanine

    Promoter

    CMV

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag9

    Plasmid

    #175539

    Purpose

    Expression of HaloTag9 at the inner membrane of mitochondria in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Cox8a-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

    Plasmid

    #171438

    Purpose

    Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-c-Jun
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge