We narrowed to 6,089 results for: cat.2

1. PIK3R5 gRNA (BRDN0001146461)

   Plasmid

   #75760

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3R5

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3R5 (PIK3R5 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. RPS6KA2 gRNA (BRDN0001148104)

   Plasmid

   #75733

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RPS6KA2 (RPS6KA2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. TEK gRNA (BRDN0001148630)

   Plasmid

   #75723

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TEK

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TEK (TEK Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. TEK gRNA (BRDN0001149392)

   Plasmid

   #75725

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TEK

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TEK (TEK Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiPGK Puro DEST p38KTRClover

   Plasmid

   #59152

   Purpose

   Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC-SUR-YR

   Plasmid

   #61768

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   Sur gene
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Tags

   Expression

   Mutation

   Promoter

   Magnaporthe oryzae ILV promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCh-KIF17*-strep

   Plasmid

   #120167

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 17 (KIF17 Human)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIF17: 1-509 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. LPAR2-Tango

   Plasmid

   #66419

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   LPAR2 (LPAR2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. sgCRY2

   Plasmid

   #197457

   Purpose

   Expresses sgRNA(CRY2 gene) in mammalian cells

   Depositor

   Han Kyoung Choe

   Article

   Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

   Insert

   hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)
   

   Use

   AAV

   Tags

   mCherry (K14E, N210D, E249G)

   Expression

   Mutation

   Promoter

   U6, hSyn

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac-M13-6His-KIF1C-GFP

    Plasmid

    #130975

    Purpose

    Expression of 6His-KIF1C-GFP in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    KIF1C-GFP (KIF1C Human)
    

    Use

    Tags

    6His and GFP

    Expression

    Insect

    Mutation

    5 silent mutations that make this construct RNAi …

    Promoter

    Polyhedrin

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

    Plasmid

    #208399

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

    Plasmid

    #208398

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    E2-Crimson

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

    Plasmid

    #208400

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865)

    Plasmid

    #49070

    Purpose

    Expresses human NKCC1 truncation mutant lacking extracellular loop #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    ECL4 deletion of amino acids 562-582 in hNKCC1 in…

    Promoter

    CMV

    Available since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Kras (G12V)-pcw107

    Plasmid

    #64602

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G12V

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-BCL6-t2A-BCL2

    Plasmid

    #135305

    Purpose

    Overexpression of multiple human genes (BCL2 and BCL6)

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Inserts

    BCL6 (BCL6 Human)
    BCL2 (BCL2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    LTR

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CL20_mEGFP_LCLAT1_GREB10

    Plasmid

    #205845

    Purpose

    Express mEGFP-tagged fusion protein, LCLAT1_GREB1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    LCLAT1_GREB1 (LCLAT1, GREB1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV

    Plasmid

    #74055

    Purpose

    retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc2, isoform C (NFATC2 Human, AS 1-686 (N terminus deleted))
    

    Use

    Retroviral

    Tags

    AVI-TEV and FLAG

    Expression

    Mammalian

    Mutation

    silent mutation A1723C in the sequence of NM_1730…

    Promoter

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)

    Plasmid

    #215759

    Purpose

    HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)

    Depositor

    Dimitrios Wagner

    Article

    Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

    Insert

    CD247-truncCD19CAR-GSG (CD19, CD247 Human)
    

    Use

    Tags

    IgG1 CH3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. UCK2_Deletion_gRNA_2

    Plasmid

    #204673

    Purpose

    Dual gRNA plasmid for UCK2 deletion

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    uridine-cytidine kinase 2 (UCK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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