We narrowed to 14,124 results for: flag

1. pHR RPS27A K107/113R-3xFlag_IRES-GFP

   Plasmid

   #198392

   Purpose

   RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   RPS27A (RPS27A Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   ubiquitination site double mutant (K107/113R)

   Promoter

   EF1A

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-TRIM24-C840W/F869D/F979A/N980A

   Plasmid

   #28144

   Depositor

   Michelle Barton

   Insert

   TRIM24 (TRIM24 Human)
   

   Tags

   FLAGX2

   Expression

   Mammalian

   Mutation

   C840W, F869D, F979A, N980A

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

   Plasmid

   #134331

   Purpose

   CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized Nme2Cas9
   

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 5'-3xFLAG Chtop-exon minimal CMV pCDNA5

   Plasmid

   #212080

   Purpose

   LR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   Chtop-exon (Chtop Mouse)
   

   Expression

   Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

   Plasmid

   #175421

   Purpose

   Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 nsp1
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA

   Plasmid

   #204547

   Purpose

   Expresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   NBR1 (NBR1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   deltaUBA

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-PGK-flag-RAP1B-V12-puro

   Plasmid

   #156168

   Purpose

   Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1B (RAP1B Bovine)
   

   Use

   Lentiviral

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Valine

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

   Plasmid

   #105801

   Purpose

   Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: FLAG-3C; ORF2: mClover3-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mHDAC7-Spliced (1-938) pEF6-Flag (MJS_015)

   Plasmid

   #73268

   Purpose

   [Edit] Expression of mouse HDAC7-S (1-938) in mammalian cells (Flag tag)

   Depositor

   Matthew Sweet

   Article

   Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

   Insert

   HDAC7 (Hdac7 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid

    #169177

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells

    Depositor

    John Diffley

    Article

    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515

    Insert

    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    

    Tags

    3xFlag-His6 (nsp5-cleavable)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV5-Flag-EPLIN alpha delta LIM

    Plasmid

    #40933

    Depositor

    Scott Maul, Elizabeth Luna

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN alpha (LIMA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    LIM domain deleted- amino acids 221-295

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pPB-CAG-3xFLAG::Fnip1-pgk-hph

    Plasmid

    #48758

    Purpose

    PiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selection

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    3xFLAG::Fnip1 (Fnip1 Mouse)
    

    Use

    Piggybac

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pLU-CMV-PL3 Flag-humanTRAF3-WT (1114)

    Plasmid

    #66922

    Purpose

    Lentiviral Expression of TRAF3

    Depositor

    Michael Karin

    Insert

    TRAF3 (TRAF3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIG-Flag-Sox9-IRES-nls-GFP

    Plasmid

    #44281

    Depositor

    Martin Cheung

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

    Insert

    Flag-Sox9 (SOX9 Chicken)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV-IE enhancer and Chicken beta-actin promoter

    Available Since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His

    Plasmid

    #83453

    Purpose

    Full-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    polycystin-1 (Pkd1 Mouse)
    

    Tags

    2xMyc, 3xFlag, and 6xHis

    Expression

    Mammalian

    Mutation

    S4144D

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV5-Flag-EPLIN alpha delta C-term

    Plasmid

    #40930

    Depositor

    Scott Maul, Elizabeth Luna

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN alpha (LIMA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 296-600

    Available Since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pTF264 (pFA6a-TEV-6xGly-3xFlag-His3MX)

    Plasmid

    #44071

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    3xFlag, 6xGly linker, ADH1 transcription terminat…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. MADR pDonor TagBFP2-3XFlag (cyto) WPRE

    Plasmid

    #129421

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-3Flag
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    3X Flag epitope tag

    Promoter

    none (3X polyA to mitigate episomal expression)

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 FLAG- SIV-MAC251-vpx-Q76A

    Plasmid

    #115843

    Purpose

    Encodes codon optimized FLAG tagged SIVMAC251-Vpx-Q76A mutant

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251-Vpx-Q76A
    

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Q76A

    Promoter

    CMV

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-Flag-Polß(D256A)-IRES-puro

    Plasmid

    #128677

    Purpose

    mammalian expression of Flag-Polß(D256A) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D256A

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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