We narrowed to 15,732 results for: LEA

1. pLV Tmem161a-V5

   Plasmid

   #175149

   Purpose

   Lentiviral expression of mouse Tmem161a-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Tmem161a (Tmem161a Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETNKI-his-3C-LIC-kan

   Plasmid

   #108703

   Purpose

   Expression of an His-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Tags

   His-3C protease cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. VQR variant

   Plasmid

   #138560

   Purpose

   Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VQR
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBXNPHM3-Nb_MsbA#1

   Plasmid

   #186428

   Purpose

   Plasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1

   Depositor

   Markus Seeger

   Article

   Galazzo et al Sci Adv. 2022 Oct 14;8(41):eabn6845. doi

   Insert

   Nanobody Nb_MsbA#1
   

   Use

   Affinity Reagent/ Antibody

   Tags

   3C cleavage site, His Tag, Maltose Binding Protei…

   Promoter

   pBAD

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV iGluf

   Plasmid

   #105833

   Purpose

   Fast indicator for extracellular glutamate imaging

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR E25D variant
   

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Changed Glu 25 to Asp

   Promoter

   CMV

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pclbw-PAGFPomp25

   Plasmid

   #58427

   Purpose

   Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   paGFP-omp25 (Synj2bp Mouse)
   

   Use

   Retroviral

   Tags

   PAGFP

   Expression

   Mammalian

   Mutation

   contains aa's 109-145 of mouse omp25

   Promoter

   cmv-bactin

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCDFNKI-his-3C-LIC-strep

   Plasmid

   #108705

   Purpose

   Expression of a His-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Tags

   His-3C protease cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MSP2372

   Plasmid

   #73196

   Purpose

   Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, Q695A, and Q926A in SpCas9

   Promoter

   CMV

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFSW Doc2alpha WT

   Plasmid

   #128812

   Purpose

   To make virus expressing Doc2alpha WT

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2alpha (Doc2a Rat)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Promoter

   hSyn (human synapsin I promoter)

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFSW Doc2beta WT

    Plasmid

    #128814

    Purpose

    To make virus expressing Doc2beta WT

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSBK.013

    Plasmid

    #187213

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT])
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTox-EGFPsite1_NGG-PAM (BPK740)

    Plasmid

    #181746

    Purpose

    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)
    

    Use

    Selection plasmid

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HOXB2 (human) HIS-tag pET

    Plasmid

    #8522

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB2 (HOXB2 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Starts at AA #2; makes protein by Western

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. Myc-Cdh1

    Plasmid

    #28127

    Depositor

    Pumin Zhang

    Article

    Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

    Insert

    fzr1 (FZR1 Human)
    

    Use

    Mouse Targeting and Retroviral

    Tags

    myc

    Expression

    Mammalian

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pRSFDuet-1_VNp-StefinA

    Plasmid

    #182403

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-stefin (CSTA Human)
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCALPS-Flag-Mili-EGFP

    Plasmid

    #212576

    Purpose

    Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

    Depositor

    Phillip Zamore

    Article

    Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

    Insert

    Mili (Piwil2 Mouse)
    

    Use

    Lentiviral

    Tags

    5x Flag and SNAP tag

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV V5-Tmem214

    Plasmid

    #175153

    Purpose

    Lentiviral expression of V5-tagged mouse Tmem214

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmem214 (Tmem214 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE2-TetOminiCMV-Oct4

    Plasmid

    #136611

    Purpose

    Dox-inducible lentiviral vector expressing mouse Oct4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLPC-mCh-ACTB-P2A-EGFP

    Plasmid

    #165158

    Purpose

    Expresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells

    Depositor

    Taekjip Ha

    Article

    Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

    Insert

    mCherry-ACTB-P2A-EGFP (ACTB Human)
    

    Use

    Retroviral

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-C1-KSENB-Nup133-mut

    Plasmid

    #87339

    Purpose

    To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes; deletion of Nup133 AA1-7

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge