We narrowed to 59,355 results for: cloning

1. pLARRYv2-EGFP

   Plasmid

   #233208

   Purpose

   Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP marker

   Depositor

   Alejo Rodriguez-Fraticelli

   Article

   Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD24-sfGFPx2

   Plasmid

   #51559

   Purpose

   Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

   Depositor

   Sankar Adhya, Francisco Malagon

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   superfolder GFP
   

   Tags

   GLESTCRHASLAVLADERRFSA and MARARA

   Expression

   Bacterial

   Mutation

   Contains additonal aa in the N and C terminus (in…

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHC79

   Plasmid

   #167551

   Purpose

   Backbone plasmid to pPM1001, pPM1002, pPM1003, pPM2212 and pPM2213

   Depositor

   Renato Morona

   Article

   Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

   Type

   Empty backbone

   Use

   Cosmid

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p663-UBC-TurboID-V5-CDK13_IDG-K

   Plasmid

   #135267

   Purpose

   Gateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   TurboID-V5-CDK13 (CDK13 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-TurboID

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pL0M-S-mKOκ-EC18154

   Plasmid

   #137077

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   mKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6_(Gluc)_INT(GenPurpClon)

   Plasmid

   #68433

   Purpose

   General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct
   

   Use

   CRISPR; Cloning vector for generating int-like co…

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6xAP-1_RE::Renilla

   Plasmid

   #178317

   Purpose

   AP-1 Renilla luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

   Insert

   AP-1 Renilla Reporter
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Mammalian

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMT-flp

   Plasmid

   #67274

   Purpose

   Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis

   Depositor

   Mark Liles

   Article

   Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

   Insert

   tetA, flp, repA101, oriR101
   

   Use

   Flp recombinase plasmid, temperature sensitive

   Tags

   His-SUMO (flp)

   Promoter

   tetA promoter of plasmid pACYC184 origin

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. p663-UBC-miniTurbo-V5-CAMK1D_IDG-K

   Plasmid

   #158167

   Purpose

   Gateway destination clone of CAMK1D (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CAMK1D (CAMK1D Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL0M-S-mTurquoise2-EC18152

    Plasmid

    #137074

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAP-BAC

    Plasmid

    #120229

    Purpose

    TAR cloning in yeast and low-copy maintenance of large constructs in E. coli

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-FLIP-puro-dsRed-GFP-miRNA

    Plasmid

    #32704

    Depositor

    Hans Clevers

    Article

    Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

    Insert

    emGFP-miRNA
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-hEF1a-MG

    Plasmid

    #175504

    Purpose

    All-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elements

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    hEF1a

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJRA107

    Plasmid

    #209340

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDHt-SK

    Plasmid

    #63615

    Purpose

    The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

    Depositor

    Seogchan Kang

    Article

    Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

    Type

    Empty backbone

    Use

    Fungal expression

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

    Plasmid

    #227774

    Purpose

    Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Insert

    loxP-dsR-loxP
    

    Use

    CRISPR

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bxb1-GA Donor_hEF1a

    Plasmid

    #229779

    Purpose

    Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL4.23-MYC

    Plasmid

    #86461

    Purpose

    A modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    MYC

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. p221z-CAS9p-TagRFP-t35s

    Plasmid

    #118386

    Purpose

    Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    CAS9p-TagRFP
    

    Use

    CRISPR

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. 7xSMAD_RE::GrRenilla

    Plasmid

    #178319

    Purpose

    SMAD GreenRenilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    SMAD GreenRenilla Reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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