We narrowed to 8,324 results for: gnal

1. pLKO shLuc/FLAG Metap2

   Plasmid

   #209316

   Purpose

   Lentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   METAP2 (METAP2 human, Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO shLYCHOS/LYCHOS WT-FLAG

   Plasmid

   #209315

   Purpose

   Lentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA)

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   LYCHOS (GPR155 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO shLYCHOS/FLAG Metap2

   Plasmid

   #209314

   Purpose

   Lentiviral construct expressing FLAG Metap2 and shRNA against LYCHOS

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   LYCHOS (GPR155 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFB-CG-LYCHOS FP>IA-HA-GFP

   Plasmid

   #199688

   Purpose

   Expression of LYCHOS FP>IA mutant in insect cells

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   LYCHOS (GPR155 Human)
   

   Use

   Tags

   10xHis, 6xHis, EGFP, HA, and HRV 3C

   Expression

   Insect

   Mutation

   F43 and P44 mutated to I and A respectively

   Promoter

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a LZ LED 4CA-FLAG

   Plasmid

   #199681

   Purpose

   Bacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper LYCHOS LED 4CA (GPR155 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   C595.604.629.638 of LYCHOS mutated to A

   Promoter

   T7 promoter

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a LZ LED Y551A-FLAG

   Plasmid

   #199680

   Purpose

   Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper LYCHOS LED Y551A (GPR155 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   Y551 of LYCHOS mutated to A

   Promoter

   T7 promoter

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-SV40-BTLAecto&tmd-PD-1icd-mGFP

   Plasmid

   #180795

   Purpose

   Expression of human BTLA extracellular domain and transmembrane domain fused to PD-1 intracellular domain followed by mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   BTLAecto&tm-PD-1int (BTLA, PDCD1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   SV40

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV x EF1a_EKAREN4(TA)-ires-blast

   Plasmid

   #167831

   Purpose

   Control sensor for EKAREN4

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4(TA)
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Cstem-Tac(5A)

   Plasmid

   #162497

   Purpose

   Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a is inserted between GFP and Tac(5A).

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   CD8a stem region and Tac full length with mutated stem region (IL2RA, CD8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The five threonine residues are mutated into alan…

   Promoter

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. MBP-rPKL S12TAG

    Plasmid

    #107155

    Purpose

    MBP-rPKL construct for production and purification of rat PKL pS12

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Serine 12 to TAG for phosphoserine insertion

    Promoter

    PBAD

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MBP-rPKL S113TAG

    Plasmid

    #107157

    Purpose

    MBP-rPKL S113TAG for production and purification of rat PKL pS113

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Serine 113 changed to TAG for phosphoserine inser…

    Promoter

    PBAD

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-Myc-TRIP12-CD

    Plasmid

    #241814

    Purpose

    Expresses Myc-tagged catalytic dead TRIP12 in mammalian cells.

    Depositor

    Ethan Lee

    Article

    Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

    Insert

    TRIP12 (TRIP12 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    catalytic dead (C2007A)

    Promoter

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Sport6-CD63-pHuji

    Plasmid

    #130902

    Purpose

    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHuji (CD63 Human, synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Gln-36 and Leu-37 in extra…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Sport6-CD9-pHluorin

    Plasmid

    #130905

    Purpose

    Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD9-pHluorin (CD9 Human, Aequorea victoria)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    phluorin inserted between Asn-50 and Asn-51 in ex…

    Promoter

    CMV

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Human, Aequorea victoria)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-Sport6-CD81-pHluorin

    Plasmid

    #130903

    Purpose

    Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD81-pHluorin (CD81 Human, aequorea victoria)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Leu-49 and Gly-50 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter

    Plasmid

    #172597

    Purpose

    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.

    Depositor

    Daniel Kiss

    Article

    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

    Insert

    myc-PDGFRA-K627R (PDGFRA Human, Synthetic)
    

    Use

    Egfp-pest_reporter

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    PDGFRA-K627R

    Promoter

    CMV

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-Lamin-A

    Plasmid

    #206027

    Purpose

    Expresses GFP-tagged Lamin A in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Lamin-A (LMNA Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSems-leader-mXFPm-IFNGR1(18-489)

    Plasmid

    #192786

    Purpose

    Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNGR1 (IFNGR1 Human)
    

    Use

    Tags

    Ig k-chain leader sequence and mXFPm

    Expression

    Mammalian

    Mutation

    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. JDW 762 (pCS2-EGFP-hsKRAS4B-G12V)

    Plasmid

    #156407

    Purpose

    pCS2 CMV expression vector driving EGFP fused human KRAS4B-G12V

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4B-G12V (KRAS Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    mutant KRAS4B-G12V

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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