We narrowed to 16,261 results for: igh

1. pBAD-Citroff1-R251

   Plasmid

   #134307

   Purpose

   Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   Citroff1 R251A
   

   Use

   Tags

   6-His tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-Citroff1-S245

   Plasmid

   #134302

   Purpose

   Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   Citroff1 S245A
   

   Use

   Tags

   6-His tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX6p1 AO7R

   Plasmid

   #137842

   Purpose

   GST tagged AO7 encoding aa 126-216 for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-hsg(NT:NT)

   Plasmid

   #138260

   Purpose

   Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   Nontargeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-T3-HypaCAS9-pA

   Plasmid

   #131467

   Purpose

   Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

   Insert

   Codon optimized HypaCas9
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOVC1_AtCRY2-PHR_X

   Plasmid

   #131180

   Purpose

   Expresses AtCRY2-PHR (Arabidopsis thaliana) followed by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   AtCRY2-PHR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOVC1_X_EcSSPB-micro

   Plasmid

   #131218

   Purpose

   Expresses EcSSPB-nano (Escherichia coli) preceded by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   EcSSPB-micro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOVC1_X_EcSSPB-nano

   Plasmid

   #131219

   Purpose

   Expresses EcSSPB-micro (Escherichia coli) preceded by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   EcSSPB-nano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOVC1_EcSSPB-micro_X

   Plasmid

   #131213

   Purpose

   Expresses EcSSPB-micro (Escherichia coli) followed by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   EcSSPB-micro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOVC1_X_AtCIB

    Plasmid

    #131194

    Purpose

    Expresses AtCIB (Arabidopsis thaliana) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    AtCIB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOVC1_X_AtCRY2-PHR

    Plasmid

    #131191

    Purpose

    Expresses AtCRY2-PHR (Arabidopsis thaliana) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    AtCRY2-PHR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOVC1_X_RsLP-LOV

    Plasmid

    #131188

    Purpose

    Expresses RsLP-LOV (Rhodobacter sphaeroides) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    RsLP-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOVC1_X_NcVVD-LOV

    Plasmid

    #131187

    Purpose

    Expresses NcVVD-LOV (Neurosporra crassa) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    NcVVD-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOVC1_X_CrPH-LOV

    Plasmid

    #131186

    Purpose

    Expresses CrPh-LOV domain (Chlamydomonas reinhardtii) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    CrPH-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOVC1_AtCIB_X

    Plasmid

    #131181

    Purpose

    Expresses AtCIB (Arabidopsis thaliana) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    AtCIB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOVC1_CrPH-LOV_X

    Plasmid

    #131175

    Purpose

    Expresses CrPh-LOV domain (Chlamydomonas reinhardtii) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    CrPH-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2+ rat MYO1D

    Plasmid

    #118419

    Purpose

    For mammalian expression of rat myosin 1D

    Depositor

    Michael Tsang

    Article

    Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

    Insert

    rat myosin 1D (Myo1d Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTrcHis-7D12Asp73TAG

    Plasmid

    #125266

    Purpose

    Expresses 7D12 anti-EGFR nanobodies with UAG codon at Asp73 for synthetic amino acid in combination with pEVOL-pAzF

    Depositor

    Angus Johnston

    Article

    Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

    Insert

    7D12 Asp73TAG
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-shP2rx4-1026

    Plasmid

    #99034

    Purpose

    Expresses a shRNA targeting the rat P2rx4 gene

    Depositor

    Amy Lasek

    Article

    Franklin et al Alcohol Clin Exp Res. 2015 Oct;39(10):20

    Insert

    P2rx4 shRNA (P2rx4 Rat)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. P0050::kaiBC S431A T432A NS1

    Plasmid

    #101837

    Purpose

    drives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locus

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    kaiBC S431A T432A with p0050 promoter
    

    Use

    Cyanobacteria cloning vector

    Tags

    Expression

    Mutation

    S431A T432A

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. P0050::kaiBC S431E T432E NS1

    Plasmid

    #101836

    Purpose

    drives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locus

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    kaiBC S431E T432E with p0050 promoter
    

    Use

    Cyanobacteria cloning vector

    Tags

    Expression

    Mutation

    S431E T432E

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21a - MPX

    Plasmid

    #89619

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Mitocondrial Tryparedoxin peroxidase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21a - EtfA

    Plasmid

    #89616

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Electron transport flavoprotein alpha subunit
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21a - EtfB

    Plasmid

    #89617

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    electron-transfer-flavoprotein, alpha polypeptide
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET21a - TDR

    Plasmid

    #89627

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    thiol dependent reductase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21a - NFDD

    Plasmid

    #89624

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    NAD/FAD dependent dehydrogenase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTRE/SP M87C448Y

    Plasmid

    #89329

    Purpose

    Inducible expression of M87 C448Y spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-460 bp deleted, C448Y

    Promoter

    Tet-responsive P tight

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTRE/SP C448Y with 5'UTR

    Plasmid

    #89325

    Purpose

    Inducible expression of C448Y spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C448Y

    Promoter

    Tet-responsive P tight

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTRE/SP M1C448Y

    Plasmid

    #89326

    Purpose

    Inducible expression of M1C448Y spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-194 bp of 5'UTR deleted, C448Y

    Promoter

    Tet-responsive P tight

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. jk63

    Plasmid

    #87011

    Purpose

    genomic Sindbis virus plasmid for use in SYNseq PLA screen

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Inserts

    CLIP-Nrx1B
    mCherry
    

    Use

    Synthetic Biology; Sindbis virus genomic plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge