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PX459_GRHL1-exon8

Plasmid

#177764

Purpose

sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

CRISPR

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-R9A-L3F6H

Plasmid

#177767

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (R9A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

R9A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H

Plasmid

#177768

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

S95A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H

Plasmid

#177769

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

K116A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H

Plasmid

#177770

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

T139A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-S374A-L3F6H

Plasmid

#177772

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S374A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

S374A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H

Plasmid

#177773

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

K592A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_sgAgo2_Nterm

Plasmid

#170920

Purpose

Expresses spCas9 and sgRNA targeting the N-terminus of Ago2 for N-terminal tagging

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo2 (Ago2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_sgAgo1_Nterm

Plasmid

#170921

Purpose

Expresses spCas9 and sgRNA targeting the N-terminus of Ago1 for N-terminal tagging

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo1 (Ago1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLV V5-Chpt1

Plasmid

#175155

Purpose

Lentiviral expression of V5-tagged mouse Chpt1

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Chpt1 (Chpt1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Dnajc16-V5

Plasmid

#175156

Purpose

Lentiviral expression of mouse Dnajc16-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Dnajc16 (Dnajc16 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Cdipt-V5

Plasmid

#175144

Purpose

Lentiviral expression of mouse Cdipt-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Cdipt (Cdipt Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSUMO R106L-CPTP

Plasmid

#170737

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

R106L-CPTP (CPTP Human)

Tags

SUMO

Expression

Bacterial

Mutation

R106L

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO K60A-CPTP

Plasmid

#170736

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

K60A-CPTP (CPTP Human)

Tags

SUMO

Expression

Bacterial

Mutation

K60A

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-CHES1L-dFN

Plasmid

#51842

Purpose

Flag-CHES1L mutant lacking the forkhead binding site and N-terminal domains

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Mutation

contains CHES1 amino acids 206-490

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1L-DBD-NLS

Plasmid

#51841

Purpose

Flag-CHES1L mutant encoding the DNA binding and NLS domains only

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag and NLS

Mutation

contains CHES1 amino acids 110-205

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1L-dC-NLS

Plasmid

#51840

Purpose

Flag-CHES1L mutant lacking the C-terminal, with remaining NLS

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag and NLS

Mutation

contains CHES1 amino acids 1-205

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1LdFC-NLS

Plasmid

#51838

Purpose

Flag-CHES1L mutant lacking the forkhead binding site and C-terminal domain, with remaining NLS

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag and NLS

Mutation

contains CHES1 amino acids 1-109

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES11L-dFC

Plasmid

#51837

Purpose

Flag-CHES1L mutant lacking the forkhead binding site and the C-terminal domains

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Mutation

contains CHES1 amino acids 1-109

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-GfaABC1D-cOpn5-T2A-EGFP

Plasmid

#237859

Purpose

The transfer plasmid for packaging AAV expressing chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of GfaABC1D promoter

Depositor

Minmin Luo

Article

Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

Insert

Opn5 (OPN5 Chicken)

Use

AAV

Promoter

GfaABC1D

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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