We narrowed to 10,337 results for: plasmids 101

1. pZS157 CRISPEY RT/Cas9

   Plasmid

   #114454

   Purpose

   Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

   Depositor

   Hunter Fraser

   Article

   Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

   Insert

   SpCas9, Ec86-RT
   

   Use

   CRISPR

   Tags

   SV40-NLS

   Expression

   Yeast

   Mutation

   S. cerevisiae codon optimized

   Promoter

   GAL1-GAL10

   Available Since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIC291

   Plasmid

   #51403

   Purpose

   Retroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Article

   Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

   Insert

   Ska1 IMAGE 3909951 (SKA1 Human)
   

   Use

   Retroviral

   Tags

   EGFP, S tag, and TEV protease site

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-eBFP2-NLS-pA

   Plasmid

   #183800

   Purpose

   AAV vector to express nuclear localized eBFP2 from CMV promoter

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

   Insert

   eBFP2-NLS
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUASTattB-dALFANb(5mut)-mGreenLantern

   Plasmid

   #219661

   Purpose

   Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-(SUMO)3

   Plasmid

   #127093

   Purpose

   Bacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   EGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   unknown

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-Lin28A

   Plasmid

   #51371

   Depositor

   Narry Kim

   Article

   Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

   Insert

   Lin28A (LIN28A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. BPK1520_puroR

   Plasmid

   #173901

   Purpose

   SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

   Depositor

   Ronald Cohn

   Article

   Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/U6

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ133B2.0

   Plasmid

   #99892

   Purpose

   Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU3

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-fDIO-mRuby3-2A-Cre

   Plasmid

   #203844

   Purpose

   Flp-dependent Cre and mRuby3 expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Cre
   

   Use

   AAV

   Tags

   mRuby3-2A

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHP138 Myc-TRF2-deltaB deltaM

    Plasmid

    #164255

    Purpose

    for generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmid

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TRF2
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    deletion of amino acids-86 and 496-542

    Promoter

    Tetracycline inducible promotor

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFGC5941-PacI-ath-STTM165/166-48nt

    Plasmid

    #84091

    Purpose

    target miRNA165/166 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM165/166
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Syn1-fDIO-mScarlet-KASH-2a-Cre

    Plasmid

    #203838

    Purpose

    Flp-dependent Cre and mScarlet-KASH expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Cre
    

    Use

    AAV

    Tags

    mScarlet-KASH-2A

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAT005

    Plasmid

    #171632

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR_PGK_SNIPR_hsNotch1 LNR, Notch2 JMD

    Plasmid

    #188374

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.13/AUG-FFLuc-3XFLAG

    Plasmid

    #127333

    Purpose

    Express 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    Firefly luciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CV40

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-FLEX-SaCas9-U6-sgSlc18a2

    Plasmid

    #124849

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-FLEX-EGFP-mir30(Scn9a)

    Plasmid

    #79672

    Purpose

    Cre-dependent knockdown of Scn9a (Nav1.7)

    Depositor

    Scott Sternson

    Article

    Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

    Insert

    EGFP (Scn9a Mouse, jellyfish)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

    Plasmid

    #124862

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. SiC-V1

    Plasmid

    #133041

    Purpose

    SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.

    Depositor

    Sriram Neelamegham

    Article

    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR_PGK_SNIPR_ALPPL2 Hinge Notch SNIPR

    Plasmid

    #188377

    Purpose

    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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