We narrowed to 18,914 results for: Tec

1. pYPQ166

   Plasmid

   #109328

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Insert

   zCas9
   

   Use

   CRISPR; Gateway compatible zcas9 entry clone

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRT498-3xOtUBD

   Plasmid

   #190088

   Purpose

   Expresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

   Depositor

   Mark Hochstrasser

   Article

   Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

   Insert

   3xOtUBD
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-blueAKAR(T/A)

   Plasmid

   #118471

   Purpose

   Negative-control mutant for blueAKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   blueAKAR(T/A)
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEL031

   Plasmid

   #137900

   Purpose

   STU2.0 SpyCas9 tRNA system for plant genome editing

   Depositor

   Yong Zhang

   Article

   Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT493

   Plasmid

   #139398

   Purpose

   Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6)
   

   Use

   Synthetic Biology

   Promoter

   Bile Acid inducible Promoter

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT494

   Plasmid

   #139399

   Purpose

   Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7)
   

   Use

   Synthetic Biology

   Promoter

   Bile Acid inducible Promoter

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT449

   Plasmid

   #139389

   Purpose

   Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)
   

   Use

   Synthetic Biology

   Promoter

   Bile Acid inducible Promoter

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT455

   Plasmid

   #139392

   Purpose

   aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate), AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate)
   

   Use

   Synthetic Biology

   Promoter

   aTC and BA inducible promoters

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. attB-puro-GA donor

   Plasmid

   #182138

   Purpose

   attB-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attB-GA-EGFP-EF1α-PuroR-T2A-TagBFP
   

   Expression

   Mammalian

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. NG-ABE8-dimer

    Plasmid

    #138497

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9 (NG)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sec61b-HA-mNG21-10

    Plasmid

    #141213

    Purpose

    Membrane Tethered Split Fluorescent Reporter for ER Retragrade

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    Sec61b-HA-mNG21-10
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDA_833

    Plasmid

    #216086

    Purpose

    Cas9 [Sp] knockout targeting CD47, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD47 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKD184

    Plasmid

    #90953

    Purpose

    Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Thiosulfate response regulator
    Superfolder GFP
    mCherry
    

    Expression

    Bacterial

    Promoter

    Bba_J23114, PltetO-1, and PphsA

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL0341 (pTarget)

    Plasmid

    #200906

    Purpose

    Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    pTarget
    

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. mApple-Annexin(A4)-12

    Plasmid

    #54867

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Annexin (A4) (ANXA4 Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

16. RBD#3L

    Plasmid

    #193020

    Purpose

    hnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

    Depositor

    Xue Gao

    Article

    Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

    Insert

    Cas13a
    

    Tags

    hnRNP A1 RRM2

    Expression

    Bacterial

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB519

    Plasmid

    #74861

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. A3A-PBE

    Plasmid

    #119768

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A and E945K in wild type …

    Promoter

    maize Ubiquitin-1

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. AA041

    Plasmid

    #215963

    Purpose

    Fragmid fragment: (Pol 2 promoter) human core (short) EF1a promoter

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    EFS_v1
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. ND4.2-Left TALE-G1397-N-DddA11

    Plasmid

    #179681

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    S1330I A1341V N1342S E1370K T1380I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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