We narrowed to 14,124 results for: flag

1. pBMN-flag beta-catenin K19R/K49R

   Plasmid

   #44751

   Depositor

   Eric Fearon

   Article

   Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

   Insert

   beta-catenin
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K19R/K49R

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)

   Plasmid

   #49063

   Purpose

   Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …

   Promoter

   CMV

   Available Since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLC-Flag-UBE2G1 sg2R-P2A-Hygro

   Plasmid

   #124299

   Purpose

   Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   UBE2G1 (UBE2G1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   several silent mutations to disrupt sgRNA targeti…

   Promoter

   CMV

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

   Plasmid

   #51249

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 980,1006 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

5. plenti-UbcP-FLAG-YWAA-pGK-HYG

   Plasmid

   #107375

   Purpose

   Lentiviral expression of FLAG-YWAA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   CRBN-Y383A/W385A

   Promoter

   UbcP

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE

   Plasmid

   #120393

   Purpose

   pAAV plasmid expressing Diras2 shRNA1 under the U6 promoter and mCherry.3XFLAG under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

   Insert

   Diras2 shRNA (Diras2 Mouse)
   

   Use

   AAV and RNAi

   Tags

   mCherry.3XFLAG

   Promoter

   U6

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-3xFLAG-Puro PAK5 K478R K469R

   Plasmid

   #167180

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R K469R

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R K469R
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)

   Plasmid

   #133788

   Purpose

   CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized St3Cas9
   

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CAG

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-mRIPK3 D161N-Flag/cHA-pTRIPZ vector

   Plasmid

   #73705

   Purpose

   lentiviral expression of mRIPK3 mutant

   Depositor

   Francis Chan

   Insert

   RIPK3 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   D161N

   Promoter

   CMV/tet

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-C1-FLAG-GFP-Ubl4A-H2(108-130)

    Plasmid

    #87203

    Purpose

    To express Ubl4A (108-130 residues) tagged with FLAG-EGFP at N- terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    UBL4A (UBL4A Human)
    

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Residues from 108-130 are inserted.

    Promoter

    CMV

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG

    Plasmid

    #29583

    Depositor

    Anna Huttenlocher

    Article

    Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80

    Insert

    phosphatidylinositol phosphate kinase type I gamma
    

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Feb. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-5X2-hDrp1(518-736)-Flag

    Plasmid

    #37244

    Depositor

    David Kashatus, Christopher Counter

    Article

    Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

    Insert

    Drp1 (DNM1L Human)
    

    Tags

    FLAG and GST

    Expression

    Bacterial

    Mutation

    contains AA 517-end

    Available Since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. MSCV N-term HA-FLAG puro BRD7 shRescue

    Plasmid

    #41936

    Depositor

    Stephen Elledge

    Article

    Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    BRD7 (BRD7 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    April 1, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pMSCV-FLAG-2xSTREP-CCND2(T280A)-Puro

    Plasmid

    #172625

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(T280A) and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr280Ala

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNIA-CEN-mVenus-FLAG-LANS1-Set2

    Plasmid

    #122003

    Purpose

    mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

    Depositor

    Brian Kuhlman

    Article

    Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

    Insert

    mVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    mVenus-FLAG

    Expression

    Yeast

    Mutation

    Set2 point mutations K538G, K539S, R549G, K550S, …

    Promoter

    ADH1

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-AVPR2-eLOV-TEVcs-FLAG-GAL4-V5

    Plasmid

    #104844

    Purpose

    b2AR-SPARK TF component

    Depositor

    Alice Ting

    Article

    Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    AVPR2-eLOV-TEVcs-FLAG-GAL4-V5
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169164

    Purpose

    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp14/nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    6His-3xFlag (nsp10 C-terminus)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-FLAG-IRES-blasticidin-Sox2 S248A

    Plasmid

    #182047

    Purpose

    expression of Sox2 mutants

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S248A

    Promoter

    CAG

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-FLAG-IRES-blasticidin-Sox2 T258A

    Plasmid

    #182048

    Purpose

    expression of Sox2 mutants

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T258A

    Promoter

    CAG

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. (363) pcDNA3.1-Nup62-Ubc-Flag-EPEA

    Plasmid

    #168098

    Purpose

    Nucleoporin Nup62 with C-terminal Ubc nanobody tag, Flag tag, and EPEA nanobody tag

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    Nup62-Ubc-Flag-EPEA
    

    Tags

    EPEA-tag, Flag-tag, and Ubc-Tag

    Expression

    Mammalian

    Mutation

    P229A, S283T on Nup62 native sequence

    Promoter

    T7/cmv

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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