We narrowed to 10,337 results for: plasmids 101

1. Nanog-2A-mCherry

   Plasmid

   #59995

   Purpose

   Inserts mCherry immediately downstream of the Nanog coding region

   Depositor

   Rudolf Jaenisch

   Article

   Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

   Insert

   Nanog (Nanog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2A-mCherry

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-mas-GRK2RH-Nluc

   Plasmid

   #158605

   Purpose

   Plasmid expressing GRK2(RH)-Nluc in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. Nanog-2A-GFP

   Plasmid

   #59994

   Purpose

   Inserts GFP immediately downstream of the Nanog coding region

   Depositor

   Rudolf Jaenisch

   Article

   Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

   Insert

   Nanog (Nanog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2A-GFP

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)/AUG-nLuc-3XFLAG

   Plasmid

   #127299

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRH623

   Plasmid

   #171633

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-non-targeting
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUASTattB-dALFANb(3mut)-mGreenLantern

   Plasmid

   #219662

   Purpose

   Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-HBB-nLuc

   Plasmid

   #175432

   Purpose

   Used to generate IVT RNA for translation extracts

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   HBB-nLuc
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV8-cPE-C

   Plasmid

   #183539

   Purpose

   Deliver a split compact Prime Editor in vivo

   Depositor

   Wen Xue

   Article

   Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

   Insert

   C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1A

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV hSyn grpLight1.2

   Plasmid

   #175174

   Purpose

   genetically encoded GRP sensor grpLight1.2

   Depositor

   Bernardo Sabatini

   Article

   Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

   Insert

   hSyn-driven grpLight1.2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAT007

    Plasmid

    #171634

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM-CAGGS-FLEX-H2B-3XHA-P2A-N2c(G)

    Plasmid

    #73477

    Purpose

    Encodes the G protein to enable transsynaptic RAV infection

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    H2B-3XHA-P2A-N2c(G)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CD4-MKII-f-hLOV1-TEVcs(ENLYFQ/M)- Gal4

    Plasmid

    #163027

    Purpose

    FLiCRE TF with fast kinetics for HEK293T expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    CD4-MKII-f-hLOV1-TEVcs-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLC133

    Plasmid

    #198639

    Purpose

    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    dCas9
    

    Use

    Tags

    3x-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV8-cPE-N

    Plasmid

    #183538

    Purpose

    Deliver a split compact Prime Editor in vivo

    Depositor

    Wen Xue

    Article

    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

    Insert

    N-terminal fragment of SpCas9 nickase (H840A)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. (SIM)5

    Plasmid

    #126947

    Purpose

    Bacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. anti-Notch2_B6-pBIOCAM5

    Plasmid

    #39347

    Depositor

    John McCafferty

    Article

    Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

    Insert

    scFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)
    

    Use

    Tags

    3xFLAG, 6xHis, and human Fc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV CAGG eGFP

    Plasmid

    #107707

    Purpose

    express eGFP in variety of cell types

    Depositor

    Troy Margrie

    Article

    Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_PGK_SNIPR_(GGS)6_SNIPR

    Plasmid

    #188344

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)6, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_(GGS)6_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. (SIM)10

    Plasmid

    #126946

    Purpose

    Bacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUW-TetO-Nanog

    Plasmid

    #40800

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV + TetO

    Available Since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only