We narrowed to 7,172 results for: 11

1. pMedium-FT-N1

   Plasmid

   #31911

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Medium-FT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

   Promoter

   CMV

   Available since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-2(KW)2-eGFP

   Plasmid

   #112736

   Purpose

   Express 2(KW)2-eGFP in E. coli

   Depositor

   Kyubong Jo

   Article

   Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

   Insert

   2(KW)2-eGFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR-TRE3G-dCas9-HaloTag

   Plasmid

   #169448

   Purpose

   expression of dCas9-HaloTag

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   dCas9-HaloTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMVTNT-T7-KPNA7

   Plasmid

   #26683

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 7 (KPNA7 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pProEx-IDE-wt

   Plasmid

   #99014

   Purpose

   Express human Insulin Degrading Enzyme (IDE) in E. coli.

   Depositor

   Wei-Jen Tang

   Article

   Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

   Insert

   insulin degrading enzyme (IDE Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   TRC

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAT253

   Plasmid

   #40943

   Depositor

   Angela Taddei

   Article

   Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

   Insert

   GFP-LacI**
   

   Use

   Tags

   GFP(S65T, V163A) and SV40 NLS

   Expression

   Yeast

   Mutation

   LacI**/LacI2G(4 mutations) (see comment below); l…

   Promoter

   His3 promoter fragment

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTM1_NP_ZEBOV

   Plasmid

   #69121

   Purpose

   Encodes for Ebola Virus Zaire NP under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

   Insert

   NP ZEBOV (NP )
   

   Use

   T7

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS2+cPLA2-mKate2

   Plasmid

   #162068

   Purpose

   Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Avian, xenopus, zebrafish

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMP2463

   Plasmid

   #107774

   Purpose

   Expression of EGFP

   Depositor

   Herman Spaink

   Article

   Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   pLacZ-EGFP in pBBR1-mcs5
   

   Use

   Tags

   pLacZ-EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   pLacZ

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pST-2K

    Plasmid

    #44564

    Purpose

    for ectopic constitutive expression of two mycobacterial proteins in one vector

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CD4-MKII-hLOV1-TEVcs(ENLYFQ/M)- Gal4

    Plasmid

    #163026

    Purpose

    FLiCRE TF for HEK293T expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    CD4-MKII-hLOV1-TEVcs-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-Nck1

    Plasmid

    #45903

    Depositor

    Louise Larose

    Article

    Latreille et al J Biol Chem. 2006 Sep 8;281(36):26633-44

    Insert

    Nck-1 (NCK1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-GFAP104-melanopsin-mCherry

    Plasmid

    #122630

    Purpose

    AAV-mediated expression of melanopsin-mCherry under the GFAP promoter

    Depositor

    Edward Boyden

    Article

    Mederos et al Glia. 2019 May;67(5):915-934. doi: 10.10

    Insert

    melanopsin-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP104

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LentiCRISPR-V2_mMSH2

    Plasmid

    #186156

    Purpose

    sgRNA targeting murine Msh2 gene

    Depositor

    David McFadden

    Article

    Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

    Insert

    Msh2_gRNA (Msh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLIC-EcTOP

    Plasmid

    #224463

    Purpose

    Expression of recombinant Escherichia coli topoisomerase I

    Depositor

    Yuk-Ching Tse-Dinh

    Article

    Tan et al Nucleic Acids Res. 2015 Dec 15;43(22):11

    Insert

    Escherichia coli TopA
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EGFP-Lyn11C32S-PIP4K2A

    Plasmid

    #202748

    Purpose

    weakly plasma membrane targeted fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    EGFP:LYN (1-11) (C3S):PIP4K2A (PIP4K2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTargeT gE

    Plasmid

    #60845

    Purpose

    Expression of the varicella zoster glycoprotein (gE) in mammalian cells

    Depositor

    Charles Grose

    Article

    Carpenter et al J Virol. 2011 Sep;85(18):9414-24. doi: 1

    Insert

    ORF68 (ORF68 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pSUB11

    Plasmid

    #169699

    Purpose

    Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
    

    Use

    Tags

    3xFLAG tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-2(KW)2-mCherry

    Plasmid

    #112738

    Purpose

    Express 2(KW)2-mCherry in E. coli

    Depositor

    Kyubong Jo

    Article

    Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

    Insert

    2(KW)2-mCherry
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pS3

    Plasmid

    #50596

    Purpose

    Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5

    Depositor

    Jay Keasling

    Article

    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

    Inserts

    aroE
    aroD
    aroB
    aroG*
    ppsA
    tktA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge