We narrowed to 8,688 results for: App

1. pGEX-4T-3-TRC8 513-664

   Plasmid

   #185352

   Purpose

   RING domain-containing GST fusion protein for ubiquitination assays.

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   TRC8 (RNF139 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 513-664 only

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET3a-UBE2J1 1-283

   Plasmid

   #185348

   Purpose

   In vitro transcription and translation in bacterial system

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   UBE2J1 (UBE2J1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cytoplasmic domain only, aa 1-283

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mTFP1-LaminB1-7

   Plasmid

   #55492

   Purpose

   Localization: Nuclear Envelope, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   LaminB1 (LMNB1 Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. mTFP1-TOMM20-N-10

   Plasmid

   #55511

   Purpose

   Localization: Outer Mitochondrial Membrane, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   TOMM20 (TOMM20 Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

5. mTFP1-Mito-7

   Plasmid

   #55500

   Purpose

   Localization: Mitochondria, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pETM33_EPS15_EH

   Plasmid

   #178475

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   EPS15_EH (EPS15 Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MAC-ACE2

   Plasmid

   #158381

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pPPAT-11AA-2XStrep

   Plasmid

   #125680

   Purpose

   expresses human PPAT protein, with first 11 residues removed, with a C-terminal 2XStrep affinity tag

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

   Insert

   PPAT (PPAT Human)
   

   Use

   Tags

   2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   the first eleven residues have been removed

   Promoter

   CMV

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MAC_C_HSP7C

   Plasmid

   #111667

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   HSP7C (HSPA8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mTFP1-Golgi-7

    Plasmid

    #55486

    Purpose

    Localization: GalT Targeting Sequence, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pmiRGLO-SERINC2-202

    Plasmid

    #117364

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR SERINC2-202
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMyc-C1-Cep123 (CCDC123/Cep89)

    Plasmid

    #69836

    Purpose

    This plasmid encodes Cep123 (CCDC123/Cep89) isoform 1 with an N-ter Myc-tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

    Insert

    Cep123 (CEP89 Human)
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EBFP2-Cx30

    Plasmid

    #69022

    Purpose

    Expresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx30 (GJB6 Human)
    

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p3F-Strep-hMIB2

    Plasmid

    #33313

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    MIB2 (MIB2 Human)
    

    Use

    Tags

    3xFlag and StrepOne

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pLenti GW-mCit-PA

    Plasmid

    #113457

    Purpose

    Lentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mCitrine-ProteinA

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mTFP1-LaminB1-10

    Plasmid

    #55493

    Purpose

    Localization: Nuclear Envelope, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pKK-TEV-FLAG

    Plasmid

    #105783

    Purpose

    Expression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MAC_N_CDK7

    Plasmid

    #111684

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    CDK7 (CDK7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LIN7A_PDZ_1

    Plasmid

    #103918

    Purpose

    Protein expression and purification of LIN7 PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    LIN7A (LIN7A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRBV12-4

    Plasmid

    #123446

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV12-4 (TRBV12-4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge