We narrowed to 16,915 results for: EMB

1. qTAG-N-Zeo-V5

   Plasmid

   #207708

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-V5 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCMU-AUTr

   Plasmid

   #61204

   Purpose

   Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   LC3(ATG8)
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TagBFP2-PIP4K2C

   Plasmid

   #202730

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mTagBFP2:PIP4K2C (PIP4K2C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-6P-1 Stu2 1-306

   Plasmid

   #38315

   Depositor

   Anthony Hyman

   Article

   Widlund et al Mol Biol Cell. 2012 Sep 19.

   Insert

   5' 918 bp from STU2 ORF (STU2 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLCS101

   Plasmid

   #209355

   Purpose

   Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mVenus fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO-FLAG-MORF4L1(iso2)

   Plasmid

   #86874

   Purpose

   To express MORF4L1 (iso2) with a FLAG tag at N-terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   MORF4L1 (MORF4L1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-UbC-eGFP-F

   Plasmid

   #71545

   Purpose

   Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.

   Depositor

   Pantelis Tsoulfas

   Insert

   eGFP-F
   

   Use

   AAV

   Tags

   contains 20- amino-acid farnesylation signal from…

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUChW nontargeting shRNA

   Plasmid

   #78522

   Purpose

   Lentiviral vector encoding shRNA and mCherry reporter

   Depositor

   David Chan

   Article

   Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

   Insert

   mCherry
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. PlayBack-CMV-Kpn1

   Plasmid

   #203302

   Purpose

   For expression of eukaryotic genes of interest with CMV promoter with Kpn1 as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK485 (3mAID-Hygro)

    Plasmid

    #214397

    Purpose

    3mAID for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    3mAID-Hygro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Gata4-GFP donor

    Plasmid

    #113115

    Purpose

    Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Gata4 donor region including homology arms and eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFBNb-cALFA

    Plasmid

    #159990

    Purpose

    For the protein expression with ALFA tag at C-terminus in insect cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDL1

    Plasmid

    #182263

    Purpose

    Gibson cloning vector for synthesis of single and double stranded RNA

    Depositor

    Daniel Lobo

    Article

    Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

    Type

    Empty backbone

    Use

    Gibson cloning vector for synthesis of single and…

    Tags

    Expression

    Mutation

    Promoter

    T3 and SP6 for sense and antisense riboprobe synt…

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAd5-B1-deltaE1-MCS

    Plasmid

    #122558

    Purpose

    Modified block 1, with deletion in E1

    Depositor

    Fred Bunz

    Article

    Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

    Insert

    Adenovirus 5 genomic region 1-3759
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Adenovirus sequences 481-3530 replaced with multi…

    Promoter

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMB1609_pRR-EGFP

    Plasmid

    #65852

    Purpose

    EGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

    Depositor

    Marc Bühler

    Article

    Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

    Insert

    split EGFP coding sequence
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-Dppa3

    Plasmid

    #13365

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    developmental pluripotency associated 3 (Dppa3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

17. RP156 pUAST EGFP:Gr21a

    Plasmid

    #59524

    Purpose

    N-terminal fusion of EGFP to Drosophila melanogaster Gr21a

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Gr21 (Gr21a Fly)
    

    Use

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pFastBac-Dual- GST-TEV-TBK1

    Plasmid

    #208875

    Purpose

    For expression in insect cells of TBK1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Human NCKX4-AS-long (pcDNA3.1+)

    Plasmid

    #75211

    Purpose

    Mammalian expression of SLC24A4

    Depositor

    Jonathan Lytton

    Article

    Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

    Insert

    SLC24A4 (SLC24A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMaCTag-07

    Plasmid

    #124786

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeonGreen
    

    Use

    Pcr template

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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