We narrowed to 9,648 results for: Ada;

1. pCDNA3-AUP1-FLAG DTM

   Plasmid

   #185337

   Purpose

   Assessing domain requirements of AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   deletion of aa 24-45, corresponding to the hairpi…

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-Solo-mStayGold-TOMM20

   Plasmid

   #227307

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-mGas8-PA

   Plasmid

   #176468

   Purpose

   Expression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

   Insert

   growth arrest specific 8 (Gas8 Mouse)
   

   Use

   Tags

   PA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDisplay-GRAB_UCN1.0

   Plasmid

   #208667

   Purpose

   Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEGFP-PI35P2

   Plasmid

   #92419

   Purpose

   Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

   Depositor

   Geert van den Bogaart

   Article

   Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

   Insert

   MCOLN1 (Mcoln1 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Synthetic gene, codon optimized for human.

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dCas12a-ABE8e-V106W

   Plasmid

   #193646

   Purpose

   Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-TadA*8e-V106W, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TadA*8e-V106W

   Promoter

   CMV

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIneoMyc UNC119Aa

   Plasmid

   #128331

   Purpose

   Mammalian expression vector of Myc-tagged human UNC119A isoform a

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

   Insert

   UNC119 (UNC119 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIneoMyc UNC119Ab

   Plasmid

   #128332

   Purpose

   Mammalian expression vector of Myc-tagged human UNC119A isoform b

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

   Insert

   UNC119 (UNC119 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.hSynapsin1.OxLight-ctr

   Plasmid

   #169793

   Purpose

   Control non-binding fluorescent reporter for orexin neuropeptides

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

   Insert

   OxLight-ctr
   

   Use

   AAV

   Tags

   Expression

   Mutation

   E54K; T111A

   Promoter

   human Synapsin-1

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-cT3G-cERP2-WT1

    Plasmid

    #192906

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of WT1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    WT1 (WT1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

    Plasmid

    #163562

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-cT3G-cERP2-NR1H2

    Plasmid

    #192919

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of NR1H2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    NR1H2 (NR1H2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX

    Plasmid

    #163554

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-Anc689-UdgX-NG-nCas9-RBMX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. retro-puro vector

    Plasmid

    #58250

    Purpose

    Retroviral expression vector encoding an empty IRES-Puromycin cassette

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

    Plasmid

    #163561

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-HF-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKK-TEV-CyOFP1

    Plasmid

    #105799

    Purpose

    Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-CyOFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-UBC-XRI-HA

    Plasmid

    #178056

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-HA
    

    Use

    AAV

    Tags

    HA-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1-shLuc.mKO2

    Plasmid

    #85224

    Purpose

    shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-GRAB_NPY1.0

    Plasmid

    #208676

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-N-Puro-sTagRFP-TUBA1B

    Plasmid

    #207769

    Purpose

    Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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