Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 6,944 results for: SIM;

Showing: 5881 - 5900 of 6944 results

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pTWIN1-His6-Ssp-Httex1-23Q-A2C-P90C

Plasmid

#114615

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 23 polyQ repeat

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-A60C

Plasmid

#114628

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 49 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-37Q-P90C

Plasmid

#114621

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 37 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q-P80C

Plasmid

#114614

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P80C; 23 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-43Q-P70C

Plasmid

#114624

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P70C; 43 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-43Q-A2C-P90C

Plasmid

#114625

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 43 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P90C

Plasmid

#114630

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 49 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C

Plasmid

#114610

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 15 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P70C

Plasmid

#114609

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P70C; 15 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P90C

Plasmid

#114620

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P80C

Plasmid

#114619

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P80C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P80C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-A60C

Plasmid

#114618

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-A60C

Plasmid

#114608

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 15 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q

Plasmid

#114612

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 23 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLuc-Optop38i-dsm

Plasmid

#89748

Purpose

Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i dark-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

C450A mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-hMLH1dn

Plasmid

#222997

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-hMLH1dn

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Deletion of residues 754-756 in MLH1 as described…

Promoter

CMV

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-LIG3

Plasmid

#215143

Purpose

Expresses mNeonGreen-LIG3 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

LIG3 (LIG3 Human)

Use

Retroviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Plasmid

#83841

Purpose

Fluorescent probe for G1/S transition and M/G1 transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

Plasmid

#216154

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with cryptic exon and C-terminal FLAG

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-PCNA

Plasmid

#215117

Purpose

Expresses mCherry-PCNA in mammalian cells from a lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 5881 - 5900 of 6944 results

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