We narrowed to 59,355 results for: cloning

1. pG-HIV-LRT

   Plasmid

   #104592

   Purpose

   Standard for qPCR

   Depositor

   Kristine Yoder

   Article

   Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

   Insert

   HIV-1 late reverse transcripts
   

   Use

   Cloning vector

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Myo 1b-myc

   Plasmid

   #135064

   Purpose

   Expression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal myc-tag.

   Depositor

   Martin Bähler

   Article

   Ruppert et al J Cell Biol. 1993 Mar;120(6):1393-403. d

   Insert

   Myo 1b with 5' UTR (Myo1b Rat)
   

   Tags

   Linker and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSA110

   Plasmid

   #59784

   Purpose

   drives mCherry in C. elegans intestinal cells

   Depositor

   Jeremy Nance

   Article

   Armenti et al Development. 2014 Dec;141(23):4640-7. do

   Inserts

   elt-2 promoter (elt-2 Nematode)
   mCherry (worm)
   unc-54 3' UTR
   

   Expression

   Bacterial

   Available Since

   Nov. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. p663-UBC-miniTurbo-V5-CDK13_IDG-K

   Plasmid

   #135266

   Purpose

   Gateway destination clone of CDK13 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK13 (CDK13 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pF hPGK mc2 IRES blast

   Plasmid

   #206239

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAC-DsRed-QF2-hsp70

   Plasmid

   #104876

   Purpose

   General purpose vector for cloning enhancer/promoter regions to drive QF2 expression; used for mosquito

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

   Insert

   QF2
   

   Expression

   Insect

   Available Since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYSD300

   Plasmid

   #212930

   Purpose

   Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   AGA1 (AGA1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Atg24 1B

   Plasmid

   #197046

   Purpose

   His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

   Depositor

   Michael Ragusa

   Article

   Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

   Insert

   Atg24
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Available Since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL0M-S-tdTomato-EC18158

   Plasmid

   #137078

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   tdTomato DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

    Plasmid

    #170182

    Purpose

    Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    mCherry
    

    Tags

    3XFlag and T2A

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQTEV-RAB17

    Plasmid

    #34812

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    RAB17 (RAB17 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgRNA(MS2)_zeo insert c-MYC

    Plasmid

    #164636

    Purpose

    sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64

    Depositor

    Günter Schneider

    Insert

    target sequence for c-Myc activation (MYC Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDN0604 (pDEST, HygR)

    Plasmid

    #210483

    Purpose

    pDEST vector with HygR marker for gene expression in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDN0603 (pDEST, NatR)

    Plasmid

    #210482

    Purpose

    pDEST vector with NatR marker for gene expression in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-TagBFP-NS

    Plasmid

    #75342

    Purpose

    Multisite gateway entry clone for adding N-terminal fusions of TagBFP

    Depositor

    Rob Parton

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    TagBFP
    

    Expression

    Mammalian

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pF CAG mc2 IRES neo

    Plasmid

    #206232

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCZGY2851

    Plasmid

    #87761

    Purpose

    Punc-17b-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in cholinergic motor neurons.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Punc-17b-PH-miniSOG(Q103L)
    

    Tags

    PH

    Expression

    Worm

    Promoter

    Punc-17b

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGAPi

    Plasmid

    #127547

    Purpose

    Gateway-based RNAi vector to clone target gene for silencing in tandem with APT transcript segment

    Depositor

    Luis Vidali

    Article

    Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00

    Type

    Empty backbone

    Use

    RNAi

    Promoter

    Ubiquitin

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p667-UBC-AKT1-V5-miniTurbo_IDG-K

    Plasmid

    #189889

    Purpose

    Gateway destination clone of AKT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p663-UBC-miniTurbo-V5-AKT1_IDG-K

    Plasmid

    #189890

    Purpose

    Gateway destination clone of AKT1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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