We narrowed to 11,245 results for: SHA

1. pET28b-Hf-RfxCas13d-His

   Plasmid

   #234480

   Purpose

   Plasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   RfxCas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Changed four alanine to valine in positions 134, …

   Promoter

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPR V2 Neo sgITGB1_3

   Plasmid

   #233250

   Purpose

   Knock out of murine ITGB1

   Depositor

   David Bryant

   Article

   Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

   Insert

   ITGB1 (Itgb1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPR V2 Neo sgITGB1_2

   Plasmid

   #233249

   Purpose

   Knock out of murine ITGB1

   Depositor

   David Bryant

   Article

   Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

   Insert

   ITGB1 (Itgb1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSG5LHARB1sgresistant

   Plasmid

   #229856

   Purpose

   RB1

   Depositor

   Maria Alvarado-Kristensson

   Article

   Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

   Inserts

   RB1 (RB1 Human)
   Rb1 (RB1 Human)
   

   Use

   Tags

   HA (N terminal on backbone)

   Expression

   Mammalian

   Mutation

   changed a bp24 to g; g bp27 to c, and c bp30 to …

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL-mGold2t

   Plasmid

   #231762

   Purpose

   Expression of mGold2t in yeast cells

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

   Promoter

   pTDH(GAP promoter)

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-multi-CRISPR-sgRipk1_#1-puro

   Plasmid

   #231984

   Purpose

   Knockout mouse Ripk1

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-multi-CRISPR-sgRipk1_#2-puro

   Plasmid

   #231983

   Purpose

   Knockout mouse Ripk1

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD)

   Plasmid

   #231233

   Purpose

   MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TAD deleted (delta1-143); numbering based on sequ…

   Promoter

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-HA-MycΔdeg1∆deg2

   Plasmid

   #231243

   Purpose

   HA-tagged MYC over-expession construct with point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of MYC degrons 1 & 2 (K355E; R356E; …

   Promoter

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

    Plasmid

    #213527

    Purpose

    Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    D90A and E92A

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSF_his6_sumo_hRNMT_RAM

    Plasmid

    #213528

    Purpose

    Expresses the human RNMT-RAM complex

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Inserts

    human RNMT (RNMT Human)
    RAM
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSF_his6_sumo_SARS_CoV2_NSP14

    Plasmid

    #213518

    Purpose

    Expresses NSP14 of SARS-CoV-2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    N-terminal His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSF_his6_sumo_OC43_NSP14

    Plasmid

    #213519

    Purpose

    Expresses NSP14 of hCoV OC43

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV OC43 NSP14 (ORF1ab hCoV OC43)
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSF_his6_sumo_NL63_NSP14

    Plasmid

    #213520

    Purpose

    Expresses NSP14 of hCoV NL63

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV NL63 NSP14 (ORF1ab hCoV NL63)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSF_his6_sumo_229E_NSP14

    Plasmid

    #213521

    Purpose

    Expresses NSP14 of hCoV 229E

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV 229E NSP14
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

    Plasmid

    #213522

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed V290 to A

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

    Plasmid

    #213523

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed N306 to K

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

    Plasmid

    #213524

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed P335 to S

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

    Plasmid

    #213525

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed P335 to H

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

    Plasmid

    #213526

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed H424 to R

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. VEGF120TAG

    Plasmid

    #200946

    Purpose

    Encodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

    Depositor

    Elizabeth Kirby

    Article

    Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

    Inserts

    VEGF120TAG (Vegfa Mouse)
    hPGK-driven Puromycin resistance
    

    Use

    Lentiviral

    Tags

    6x HIS

    Expression

    Mutation

    amber stop codon 101 bp from ATG

    Promoter

    Ef1a and hPGK

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. VEGF164TAG

    Plasmid

    #200947

    Purpose

    Encodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

    Depositor

    Elizabeth Kirby

    Article

    Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

    Inserts

    VEGF164TAG (Vegfa Mouse)
    hPGK-driven Puromycin resistance
    

    Use

    Lentiviral

    Tags

    6x HIS

    Expression

    Mutation

    amber stop codon 101 bp from ATG

    Promoter

    Ef1a and hPGK

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CIRTS-YTHDF2-Malat1

    Plasmid

    #216858

    Purpose

    CIRTS RNA targeting system for targeted knockdown of m6A-containing Malat1 at the synapse. CIRTS-YTHDF2-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

    Insert

    CIRTS-YTHDF2-Calm3-U6-Malat1 gRNA
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Syn1, U6

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CIRTS-FTO-Malat1

    Plasmid

    #216860

    Purpose

    CIRTS RNA targeting system for targeted removal of m6A on Malat1 at the synapse. CIRTS-FTO-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

    Insert

    CIRTS-FTO-Calm3-U6-Malat1 gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Syn1, U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-U6-sgCTG-CMV-GFP

    Plasmid

    #216734

    Purpose

    AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    sgCTG
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-nEF-SpCas9D10A nickase

    Plasmid

    #216736

    Purpose

    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    EF-1-alpha core

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CILK1-R272Q

    Plasmid

    #214911

    Purpose

    expression of the mutant variant of CILK1

    Depositor

    Pavel Krejčí

    Article

    Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

    Insert

    CILK1 (CILK1 Human)
    

    Use

    Tags

    Myc-FLAG

    Expression

    Mammalian

    Mutation

    R272Q substitution

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CILK1-E80K

    Plasmid

    #214910

    Purpose

    expression of the mutant variant of CILK1

    Depositor

    Pavel Krejčí

    Article

    Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

    Insert

    CILK1 (CILK1 Human)
    

    Use

    Tags

    Myc-FLAG

    Expression

    Mammalian

    Mutation

    E80K substitution

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lenti-sgRNA-Cre-GpNLuc-sgmATM

    Plasmid

    #208392

    Purpose

    Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ATM gene.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgATM (Atm Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUb FLAG-Mts D85N

    Plasmid

    #208403

    Purpose

    Expresses FLAG-tagged Drosophila mts protein with D85N mutation

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    microtubule star (mts Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    D85N

    Promoter

    Ubi-p63e

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge