We narrowed to 10,393 results for: plasmids 101

1. DIO-EF1a-FLEX-H2B-3XHA-P2A-N2c(G)

   Plasmid

   #73478

   Purpose

   Encodes the G protein to enable transsynaptic RAV infection

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   H2B-3XHA-P2A-N2c(G)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)/UUG-nLuc-3XFLAG

   Plasmid

   #127302

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJRH056

   Plasmid

   #171637

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-tdTomato
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. RFP-(SIM)1

   Plasmid

   #127094

   Purpose

   Bacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   mCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
   

   Tags

   mCherry

   Expression

   Bacterial

   Promoter

   unknown

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PLKO.1-CAPRIN1-3'UTR

   Plasmid

   #136055

   Purpose

   CAPRIN1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCACGTTAGTGTCACAAATT)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   CAPRIN1 (CAPRIN1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSO221 Phis-72 BirA GFP

   Plasmid

   #45100

   Depositor

   Steven Henikoff

   Article

   Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

   Inserts

   BirA::GFP
   unc-119 (unc-119 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   his-72 and unc-119

   Available Since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIC292

   Plasmid

   #51405

   Purpose

   Retroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Article

   Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

   Insert

   Ska2 IMAGE 4685972 (SKA2 Human)
   

   Use

   Retroviral

   Tags

   EGFP, S tag, and TEV protease site

   Expression

   Mammalian

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJM160

   Plasmid

   #134169

   Purpose

   Bacterial expression of RB3 stathmin-like domain (SLD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   RB3-SLD (STMN4 Human)
   

   Expression

   Bacterial

   Mutation

   residues 49-189

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-2A-GFP-G3BP2(1)

   Plasmid

   #136060

   Purpose

   G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP2 (G3BP2 Human)
   

   Expression

   Mammalian

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. DNA-CARzeta-IRESpuro

    Plasmid

    #89345

    Purpose

    2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    DNA-CARzeta
    

    Use

    Lentiviral

    Tags

    SNAPf tag

    Expression

    Mammalian

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. POLArIS_NbVim-cp10GFP

    Plasmid

    #171020

    Purpose

    Expresses NbVim-cp10GFP#2 in mammalian cells

    Depositor

    Sumio Terada

    Article

    Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

    Insert

    anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
    

    Tags

    cp10GFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAT024

    Plasmid

    #171636

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BFP
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G)

    Plasmid

    #73475

    Purpose

    Encodes the G protein to enable transsynaptic RAV infection

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    H2B-GFP-P2A-N2c(G)
    

    Use

    AAV

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pYPQ133C2.0

    Plasmid

    #99893

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    OsU6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-hSyn-Gαi*-BERKY3

    Plasmid

    #158428

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. PLKO.1-CAPRIN1-CDS

    Plasmid

    #136054

    Purpose

    CAPRIN1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CCTCAGCAGAACACTGGATTT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    CAPRIN1 (CAPRIN1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ131C2.0

    Plasmid

    #99886

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    OsU6

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ132C2.0

    Plasmid

    #99889

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    OsU6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-hSyn-Gαq*-BERKY1

    Plasmid

    #158430

    Purpose

    BRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-YFP-GRK2(RH)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. C-BLa 2.0

    Plasmid

    #92060

    Purpose

    The C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.

    Depositor

    Dieter Langosch

    Article

    Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

    Insert

    C-BLa 2.0 fusion protein
    

    Tags

    Flag (DYKDHDG)

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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