We narrowed to 10,433 results for: yeast

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pSKT001

Plasmid

#218594

Purpose

Express degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

UbiY degradation tag and ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1357

Plasmid

#218595

Purpose

Express Pex11 and the degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex11

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKS1461

Plasmid

#218587

Purpose

Express peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPP

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Erg12

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p500-Erg6-V5-APEX2-URA

Plasmid

#207070

Purpose

Expressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Erg6

Tags

V5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-OM14-V5-APEX2-Ura

Plasmid

#207073

Purpose

Expressing OM14-V5-APEX2. Marker for mitochondrial outer membrane.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

OM14

Tags

V5-APEX2

Expression

Yeast

Promoter

pOM14

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-100

Plasmid

#219408

Purpose

Reporter gene (4 operators) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x4-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-422

Plasmid

#219410

Purpose

Reporter gene (1 operator) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x1-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-424

Plasmid

#219412

Purpose

Reporter gene (3 operator) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x3-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-095

Plasmid

#219407

Purpose

Reporter gene (4 operators) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(13-6 op)x4-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKL1-423

Plasmid

#219411

Purpose

Reporter gene (2 operator) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x2-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Cox4-V5-APEX2-Ura

Plasmid

#207061

Purpose

Expressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

V5-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Cox4-GFP-APEX2-Ura

Plasmid

#207062

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

GFP-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pADH3-Snf7-4V5-APEX2-URA

Plasmid

#207065

Purpose

Moderate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Snf7

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pERG6-Pex3-4V5-APEX2-URA

Plasmid

#207068

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pLSP1-Pex3-4V5-APEX2-URA

Plasmid

#207069

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pLSP1

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-pTPI1-Emc1-GFP-APEX2-Ura

Plasmid

#207074

Purpose

Expressing Emc1-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Emc1

Tags

GFP-APEX2

Expression

Yeast

Promoter

pTPI1

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pTPI1-Cox4-GFP-APEX2-Ura

Plasmid

#207075

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

GFP-APEX2

Expression

Yeast

Promoter

pTPI1

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.F03R

Plasmid

#198664

Purpose

Easy-MISE toolkit pEM-plasmid containing mCherry coding sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

mCherry

Use

Synthetic Biology

Expression

Yeast

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.F03L

Plasmid

#198663

Purpose

Easy-MISE toolkit pEM-plasmid containing mCherry coding sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

mCherry

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.F01R

Plasmid

#198660

Purpose

Easy-MISE toolkit pEM-plasmid containing GFP coding sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

GFP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.F01L

Plasmid

#198659

Purpose

Easy-MISE toolkit pEM-plasmid containing GFP coding sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

GFP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.A02R

Plasmid

#198658

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with FL protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.A02L

Plasmid

#198657

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with BF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.A01R

Plasmid

#198656

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.A01L

Plasmid

#198655

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPpT4- HTA1prom-TIR1-FLAG

Plasmid

#189725

Purpose

Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

HTA1

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPpT4- TEF2prom-TIR1-FLAG

Plasmid

#189726

Purpose

Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

TEF2

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-URA-GAL1-fCLIP-GS

Plasmid

#188939

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fCLIP-GS

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-URA-GAL1-fSNAP-GS

Plasmid

#188938

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fSNAP-GS

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-URA-GAL1-DHFR-3xHA

Plasmid

#188934

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-DHFR-3xHA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge