We narrowed to 7,531 results for: Ski;

1. S1 1029 CP APOBEC3 no UGI

   Plasmid

   #135352

   Purpose

   Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   rAPOBEC3-nSpyCas9 aa 1029
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. S1 1029 CP ABEMax strategy 2

   Plasmid

   #135358

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   WT Tada-nSpyCas9 aa 1029-evolved Tada
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBOB-N-3HA-GK_v2

   Plasmid

   #134277

   Purpose

   Lentivector encoding 3XHA-tagged GK (isoform 2)

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk (Gk Mouse)
   

   Use

   Lentiviral

   Tags

   3X HA

   Expression

   Mammalian

   Mutation

   553 amino acids, isoform 2

   Promoter

   CMV

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1/NDUFS2 _34-66/myc-His

   Plasmid

   #127495

   Purpose

   Expresses myc-tagged _34-66 NDUFS2 in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 _34-66 (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   NDUFS2 _34-66

   Promoter

   CMV

   Available Since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/NDUFS2 E57A/myc-His

   Plasmid

   #127497

   Purpose

   Expresses myc-tagged NDUFS2/E57A in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 E57A (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   NDUFS2 E57A

   Promoter

   CMV

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1/NDUFS2 T58A/myc-His

   Plasmid

   #127498

   Purpose

   Expresses myc-tagged NDUFS2/T58A in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 T58A (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   NDUFS2 T58A

   Promoter

   CMV

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/NDUFS2 H60A/myc-His

   Plasmid

   #127499

   Purpose

   Expresses myc-tagged NDUFS2/H60A in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 H60A (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   NDUFS2 H60A

   Promoter

   CMV

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/NDUFS2 W61A/myc-His

   Plasmid

   #127500

   Purpose

   Expresses myc-tagged NDUFS2/W61A in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 W61A (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   NDUFS2 W61A

   Promoter

   CMV

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/NDUFS2 K62A/myc-His

   Plasmid

   #127501

   Purpose

   Expresses myc-tagged NDUFS2/K62A in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 K62A (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   NDUFS2 K62A

   Promoter

   CMV

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/NDUFS2 P63A/myc-His

    Plasmid

    #127502

    Purpose

    Expresses myc-tagged NDUFS2/P63A in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 P63A (NDUFS2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 P63A

    Promoter

    CMV

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJET1.2_Bod1l_Anti-Sense

    Plasmid

    #124444

    Purpose

    Plasmid for anti-sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)
    

    Use

    In situ probe

    Promoter

    T7

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJET1.2_Gli3_Sense

    Plasmid

    #124439

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    GLI-Kruppel family member GLI3 (Gli3 Mouse)
    

    Use

    In situ probe

    Promoter

    T7

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-TRC.mKO2_shHRASLS.2

    Plasmid

    #110324

    Purpose

    TRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    HRASLS (PLAAT1 Human, Homo sapiens)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-TRC.mKO2_shHRASLS.1

    Plasmid

    #110323

    Purpose

    TRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    HRASLS (PLAAT1 Human, Homo sapiens)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP Kind2 S159/181/666E

    Plasmid

    #105314

    Purpose

    To study altered downstream functions of phospholylation-site mimetic kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666E

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_ZNF597_WT

    Plasmid

    #82894

    Purpose

    Gateway Donor vector containing ZNF597, part of the Target Accelerator Plasmid Collection.

    Depositor

    Matthew Meyerson, David Root, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    ZNF597 (ZNF597 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid

    #185910

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE-neomycin-PC-MYC

    Plasmid

    #184550

    Purpose

    Retroviral vector to express human PC with MYC tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid

    #73497

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB-P2A-mCherry
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.2 TDP-43 YFP

    Plasmid

    #84911

    Purpose

    expression of TDP-43 YFP in mammalian cells

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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