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We narrowed to 18,759 results for: nls

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Showing: 11921 - 11960 of 18759 results

  3. pLenti-nSpCas9-NoCBE

    Plasmid
    #209038
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209038-plasmid-map-sequence-id-413620

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  4. pLenti-nSpCas9-BEACON2

    Plasmid
    #209041
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    BEACON2-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209041-plasmid-map-sequence-id-419488

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  5. pLenti-nSpCas9-TadA8.20m

    Plasmid
    #209045
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TadA8.20m-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209045-plasmid-map-sequence-id-413618

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  11. apt-ABE8.20

    Plasmid
    #219938
    Purpose
    Mammalian expression of apt-ABE8.20 protein (TadA8.20_32aa-linker_Com)
    Depositor
    Alexis Komor
    Publication
    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA8.20_32-aa_Com
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219938-plasmid-map-sequence-id-450044

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  16. AAVS1-iPE2-Hygro

    Plasmid
    #214019
    Purpose
    inducible PE2 system for controllable prime editing; this plasmid is used to insert PE2 prime editor in one allele of AAVS1 locus
    Depositor
    Ting Zhou
    Publication
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2
    Expression
    Mammalian
    Promoter
    TRE-tight
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214019-plasmid-map-sequence-id-429369

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  18. pSCL758

    Plasmid
    #219504
    Purpose
    Express Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
    Depositor
    Seth Shipman
    Publication
    Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
    Expression
    Mammalian
    Promoter
    H1
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219504-plasmid-map-sequence-id-487657

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  19. AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-miR122 TS

    Plasmid
    #209783
    Purpose
    Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter, incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)
    Depositor
    Yuxuan Guo
    Publication
    Yang et al Circulation. 2024 May 28;149(22):1778-17
    Insert
    miR122 ts
    Use
    AAV
    Tags
    HA
    Promoter
    cTNT
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    209783-plasmid-map-sequence-id-415445

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  23. hPLD3-sgRNA-Cas9_mcherry

    Plasmid
    #199342
    Purpose
    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Promoter
    human U6
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199342-plasmid-map-sequence-id-390619

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  25. pYPQ265E6

    Plasmid
    #213469
    Purpose
    Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing
    Depositor
    Yiping Qi
    Publication
    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:
    Insert
    TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
    Use
    CRISPR; Gateway compatible tada-cda-32aa linker-z…
    Tags
    No
    Expression
    Plant
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213469-plasmid-map-sequence-id-426729

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  26. pYPQ265E8

    Plasmid
    #213471
    Purpose
    Gateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing
    Depositor
    Yiping Qi
    Publication
    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:
    Insert
    TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI
    Use
    CRISPR; Gateway compatible tada-cdd_v106w-32aa li…
    Tags
    No
    Expression
    Plant
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213471-plasmid-map-sequence-id-426730

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  29. pCMV-dCas12a-A3A

    Plasmid
    #193638
    Purpose
    Expression of base editor dCas12a-A3A in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a-hA3A, EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    193638-plasmid-map-sequence-id-421920

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  34. ATM N-terminal sgRNA

    Plasmid
    #207089
    Purpose
    pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.
    Depositor
    Jens Schmidt
    Publication
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATCATTAAGTACTAGACTCA
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    207089-plasmid-map-sequence-id-431774

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  35. AA184

    Plasmid
    #216047
    Purpose
    Fragmid fragment: (C' terminus) for prime editing; using PEmax
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1
    Use
    Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216047-plasmid-map-sequence-id-431531

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  36. AA058

    Plasmid
    #216040
    Purpose
    Fragmid fragment: (C' terminus) for prime editing
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLV-RT_v1.1; SV40NLS_v1.3
    Use
    Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216040-plasmid-map-sequence-id-431496

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  37. pMV_AA091

    Plasmid
    #216106
    Purpose
    CRISPRa, gRNA expression with modified trRNA to recruit activators via PP7 tags (guide only)
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    gRNA expression with modified trRNA to recruit activators via PP7 tags
    Use
    CRISPR and Lentiviral; Assembled vector
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216106-plasmid-map-sequence-id-431410

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  40. AAVS1 EGFP-Sec61B

    Plasmid
    #207553
    Purpose
    Homologous recombination donor to integrate and expression cassette for EGFP-Sec61B in the AAVS1 locus of human cells
    Depositor
    Jens Schmidt
    Publication
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TET inducible EGFP-Sec61B
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    Endogenous
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    207553-plasmid-map-sequence-id-431006

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Showing: 11921 - 11960 of 18759 results