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Lenti_stgRNA-Puro

Plasmid

#164124

Purpose

Backbone for lentiviral stgRNA library construction (library 1 and 2)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPD150 ZF43x12-Spaced-Far_EYFP

Plasmid

#138895

Purpose

EYFP reporter vector for the ZF43x12-Spaced-Far promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced-Far

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x12-Spaced-Far

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPD147 ZF43x8-Spaced-Far_EYFP

Plasmid

#138893

Purpose

EYFP reporter vector for the ZF43x8-Spaced-Far promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x8-Spaced-Far

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x8-Spaced-Far

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPD145 ZF43x4-Spaced-Far_EYFP

Plasmid

#138891

Purpose

EYFP reporter vector for the ZF43x4-Spaced-Far promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x4-Spaced-Far

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x4-Spaced-Far

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPD144 ZF43x2-Spaced-Far_EYFP

Plasmid

#138890

Purpose

EYFP reporter vector for the ZF43x2-Spaced-Far promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x2-Spaced-Far

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x2-Spaced-Far

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPD140 ZF43x12-Spaced-Mid_EYFP

Plasmid

#138889

Purpose

EYFP reporter vector for the ZF43x12-Spaced-Mid promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x12-Spaced-Mid

Available Since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pPD137 ZF43x8-Spaced-Mid_EYFP

Plasmid

#138887

Purpose

EYFP reporter vector for the ZF43x8-Spaced-Mid promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x8-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x8-Spaced-Mid

Available Since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pPD135 ZF43x4-Spaced-Mid_EYFP

Plasmid

#138885

Purpose

EYFP reporter vector for the ZF43x4-Spaced-Mid promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x4-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x4-Spaced-Mid

Available Since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pPD134 ZF43x2-Spaced-Mid_EYFP

Plasmid

#138884

Purpose

EYFP reporter vector for the ZF43x2-Spaced-Mid promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x2-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x2-Spaced-Mid

Available Since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2082

Plasmid

#155162

Purpose

Expresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(MlaFΔ[248-269])-(6xHis-TEV-MlaB)

Expression

Bacterial

Mutation

deleted amino acids 248-269 in MlaF

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1514

Plasmid

#155147

Purpose

Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1965

Plasmid

#155152

Purpose

Expresses MlaF(1-246)-(6 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1966

Plasmid

#155153

Purpose

Expresses MlaF(1-246)-(10 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1967

Plasmid

#155154

Purpose

Expresses MlaF(1-246)-(14 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAG369-lyn11-redFAST

Plasmid

#160356

Purpose

Expresses Lyn11-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAG361-lyn11-greenFAST

Plasmid

#160352

Purpose

Expresses Lyn11-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-greenFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pUC_h7SK M15 (CUA)

Plasmid

#154784

Purpose

plasmid with single copy of Mma PylT M15 mutant (CUA anticodon)

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylT

Expression

Mammalian

Mutation

G10A, G14U, G16U, C20U, C52A

Promoter

h7SK

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCR3.1-CREM2 luc

Plasmid

#106460

Purpose

luciferase reporter, CMV promoter (CRE site adjacent to NFkB site mutated)

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

luciferase reporter, CMV promoter (CRE site adjacent to NFkB site mutated)

Use

Luciferase

Expression

Mammalian

Mutation

CRE site adjacent to NFkB site mutated

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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P313-FBP_8

Plasmid

#139699

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing H3H

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp, pAtuMas:Nn Luz:trbcSE9

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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P302-FBP_1

Plasmid

#139692

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

p35S_Short_TMV 5':AsNpga:tAtug7, AtUbi10:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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