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pCS5

Plasmid

#55750

Purpose

Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Publication

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Expression

Bacterial

Mutation

Residues 59-264 of KanR

Promoter

T7-lac

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP3K15

Plasmid

#23499

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K15 (MAP3K15 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-GALK1

Plasmid

#23743

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GALK1 (GALK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCheY Tc delta C

Plasmid

#13520

Depositor

James McKerrow

Publication

Eakin et al J Biol Chem. 1993 Mar 25. 268(9):6115-8.

Insert

cruzain

Tags

CheY

Expression

Bacterial

Mutation

Truncated variant: stop codon after Gly212

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only
mEGFP-2xbiRhoBAST

Plasmid

#196349

Purpose

Expression of mEGFP mRNA with 2xbiRhoBAST in 3'UTR

Depositor

Murat Sunbul

Publication

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mEGFP-2xbiRhoBAST

Expression

Mammalian

Promoter

CMV

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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ADD1

Plasmid

#155397

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ADD1 (ADD1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-rtTA-zeo

Plasmid

#183233

Purpose

Lentiviral vector expressing the rtTa gene

Depositor

Jorge Ferrer

Publication

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

rtTA-T2A-Zeo

Use

Lentiviral

Promoter

UBC

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-FRT-GFP-FRT-u(M)Cas9

Plasmid

#127388

Purpose

Cas9 inducible by removal of a GFP flip-out cassette

Depositor

Michael Boutros

Publication

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

FRT-GFP-FRT-uORF(M)Cas9

Use

CRISPR

Expression

Insect

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pES134

Plasmid

#127551

Purpose

Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

Depositor

Emmanuele Severi, Gavin Thomas

Publication

Wu et al Microbiology (Reading). 2024 Mar;170(3).

Insert

F3-flanked spectinomycin resistance cassette

Use

Synthetic Biology

Expression

Bacterial

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shWRN1

Plasmid

#125781

Purpose

constitutive expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1 (WRN Human)

Use

RNAi

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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10xUAS-GFP-DD

Plasmid

#104989

Purpose

Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

Depositor

Jing-W Wang

Publication

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized GFP (GFP-DD)

Tags

DD

Expression

Insect

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC

Plasmid

#71812

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Publication

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC

Expression

Insect

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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BASIC_7_J23101-RBS34-mCherry-B0015

Plasmid

#68141

Purpose

storage vector for BASIC part: RFP expression cassette

Depositor

Geoffrey Baldwin

Publication

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

J23101-RBS34-mcherry-B0015

Expression

Bacterial

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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hIRmut-GFP

Plasmid

#22397

Depositor

Joseph Bass

Publication

Ramos et al Proc Natl Acad Sci U S A. 2007 Jun 19. 1

Insert

IRmut

Tags

GFP

Expression

Mammalian

Mutation

changed Leu 62 to Proline

Available since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-5X-1-DJ1-D149A

Plasmid

#29351

Depositor

Mark Cookson

Publication

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Asp149Ala

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-YSK4

Plasmid

#23616

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

YSK4 (YSK4 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pTn7-SCOUT21.RB

Plasmid

#201007

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-mCherry-TagBFP, GmR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

mcherry
TagBFP

Expression

Bacterial

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pS848∙CsR

Plasmid

#199240

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Publication

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Gm resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+18)

Plasmid

#199162

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Publication

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+18)

Tags

MGHHHHHHGGA

Mutation

E16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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CSTF1

Plasmid

#155485

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CSTF1 (CSTF1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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