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pmiR-96 cluster promoter

Plasmid

#62517

Purpose

microRNA 183-96-182 cluster promoter luciferase plasmid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2014 Mar;42(5):2988-9

Insert

promoter of microRNA-183-96-182 cluster

Use

Tags

RenSP (optimized renilla luciferase gene)

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Drosha218-1374

Plasmid

#62523

Purpose

N-terminal aa1-217 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-217 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-217

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Drosha270-1374

Plasmid

#62524

Purpose

N-terminal aa1-269 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-269 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS300A

Plasmid

#62526

Purpose

Serine 300 of Drosha is mutated to alanine

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 300 changed to alanine (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 300 to alanine

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS302A

Plasmid

#62527

Purpose

Serine 302 of Drosha is mutated to alanine

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 302 changed to alanine (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 302 to alanine

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-DroshaK382R

Plasmid

#62519

Purpose

Lysine 382 of Drosha was mutated to arginine

Depositor

Bharat Ramratnam

Article

Tang et al PLoS One. 2013 Aug 29;8(8):e72503. doi:

Insert

human Drosha with lysine 382 changed to arginine (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed lysine 383 to arginine

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pCW-Cdt1-mCherry-Puro

Plasmid

#193763

Purpose

Doxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1-mCherry (CDT1 Human)

Use

Lentiviral

Tags

WT

Expression

Mutation

human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

Promoter

TRE

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-Cdt1ΔPIP-mCherry-Puro

Plasmid

#193765

Purpose

Doxycyline-inducible (TetOn) human Cdt1ΔPIP (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1ΔPIP-mCherry (CDT1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

PIP degron removed (a.a.1-19 removed)

Promoter

TRE

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP

Plasmid

#170327

Purpose

Double-floxed inverse Orientation HA and IRES-YFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P-rTetO-EGFP-T2A-RPL22-3xHA

Plasmid

#170326

Purpose

Tetracycline-inducible expression of HA and EGFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-mCherry-hCdt1(1-100)ΔCy

Plasmid

#193759

Purpose

Expresses N-CRL4Cdt2 reporter(mCherry-hCdt1(1-100)ΔCy)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

mCherry-hCdt1(1-100)ΔCy (CDT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

Promoter

EF1a

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-DroshaS300/302A

Plasmid

#62530

Purpose

Serines 300 and 302 of Drosha are mutated to alanines

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serines 300 and 302 changed alanines (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serines 300 and 302 to alanines

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

Plasmid

#170317

Purpose

Expresses HA and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

Plasmid

#170320

Purpose

Expresses HA and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo

Plasmid

#170321

Purpose

Expresses HA and mCherry tagged RPL22, neomycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

Plasmid

#170318

Purpose

Expresses FLAG and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo

Plasmid

#170319

Purpose

Expresses HA and EGFP tagged RPL22, neomycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-Cdt1ΔCy-mCherry-Puro

Plasmid

#193764

Purpose

Doxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1ΔCy-mCherry (CDT1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Cy motif (a.a. 68-70) mutated to alanine, a.a. 11…

Promoter

TRE

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
P-Ef1a-RPL22-3XFlag-P2A-mCherry-T2A-Puro

Plasmid

#170322

Purpose

Expresses FLAG and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P-rTetO-mCherry-T2A-RPL22-1xV5

Plasmid

#170325

Purpose

Tetracycline-inducible expression of V5 and mCherry tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge