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We narrowed to 157,564 results for: HTT

Showing: 41 - 60 of 157564 results

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pRS416 CUP1pro-Htt103QdeltaP161-170-YFP

Plasmid

#224876

Purpose

Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 10 amino acids of the proline-rich region (161-170) tagged with YFP using copper.

Depositor

Paul Herman

Insert

HTT103QdeltaP161-170 (HTT Human)

Use

Tags

YFP

Expression

Yeast

Mutation

delta161-170

Promoter

CUP1

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-HTT-SVA

Plasmid

#202833

Purpose

pGL3 luciferase reporter with HTT SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

HTT intronic SVA

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1

Plasmid

#190902

Purpose

AAV vector expressing thew GFP reporter gene and human sgHTT

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

GFP and sgHTT (HTT Human)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

EFS and U6

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Htt171-66Q-myc-WPRE

Plasmid

#107934

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons

Depositor

Johan Jakobsson

Article

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

Exon 1 of mutant HTT, 66 polyQ repeats (HTT Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSynapsin

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Htt171-18Q-myc-WPRE

Plasmid

#107936

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neurons

Depositor

Johan Jakobsson

Article

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

Exon 1 of wild type HTT (HTT Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSynapsin

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-43Q-P90C

Plasmid

#114626

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Use

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 43 polyQ repeat

Promoter

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-15Q-P90C

Plasmid

#114611

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Use

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 23 polyQ repeat

Promoter

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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SIN-cPPT-H1-sgHTT1-PGK-mCherry-WPRE

Plasmid

#87885

Purpose

To produce lentiviral vector for HTT editing

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

shHTT1, cherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

H1, PGK

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q

Plasmid

#114617

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Use

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 23 polyQ repeat

Promoter

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-43Q-A2C

Plasmid

#114622

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C cysteine mutation and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Use

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 43 polyQ repeat

Promoter

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q

Plasmid

#114627

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Use

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 49 polyQ repeat

Promoter

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-49Q-P90C

Plasmid

#114631

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Use

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 49 polyQ repeat

Promoter

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

Plasmid

#225228

Purpose

Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

Depositor

Urartu Seker

Article

Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

Inserts

Aga2
Htt (25Q)

Use

Tags

HA Tag and c-myc Tag

Expression

Yeast

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-Htt(46Q)-c_myc

Plasmid

#225229

Purpose

Yeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.

Depositor

Urartu Seker

Article

Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

Inserts

Aga2
Htt (46Q)

Use

Tags

HA Tag and c-myc Tag

Expression

Yeast

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9

Plasmid

#190903

Purpose

AAV-KamiCas9 vector expressing expressing thew GFP reporter gene and sgHTT and sgCas9

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

GFP, sgHTT and sgCas9 (HTT Human)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

EFS and U6

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-37Q-P90C

Plasmid

#114621

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Use

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 37 polyQ repeat

Promoter

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q

Plasmid

#114612

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Use

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 23 polyQ repeat

Promoter

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q-A2C-A60C

Plasmid

#114613

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Use

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 23 polyQ repeat

Promoter

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-43Q-A2C-A60C

Plasmid

#114623

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Use

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 43 polyQ repeat

Promoter

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P90C

Plasmid

#114606

Purpose

Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 42 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Use

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

dNt17 (deletion of first 17 amino acids), Q18C, P…

Promoter

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Showing: 41 - 60 of 157564 results

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