We narrowed to 126 results for: PYE

1. pYES2.1-pomMet17

   Plasmid

   #107453

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   Met17 (met17 Fission Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYes2-AtPCS1

   Plasmid

   #49766

   Purpose

   Expresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces Cerevisiae

   Depositor

   Julian Schroeder

   Article

   Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

   Insert

   AtPCS1 (PCS1 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pYES2.1-ScDcr1deltaN

   Plasmid

   #32038

   Depositor

   David Bartel

   Article

   Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

   Insert

   Drc1(111-610)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pYea5.1_Notch1-L24_sfGFP

   Plasmid

   #207865

   Purpose

   Yeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Suc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein
   

   Use

   Tags

   Strep-TagII

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYES2.1-ScDcr1deltaC

   Plasmid

   #32037

   Depositor

   David Bartel

   Article

   Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

   Insert

   Drc1(17-356)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pYES2.1-ScDcr1

   Plasmid

   #32036

   Depositor

   David Bartel

   Article

   Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

   Insert

   Drc1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pYES2.1-TRP1URA3

   Plasmid

   #11008

   Depositor

   Francisco Malagon

   Insert

   TRP1URA3 fusion
   

   Use

   Tags

   V5 and his6

   Expression

   Bacterial and Yeast

   Mutation

   ORF-TRP1 fused to ORF-URA without stop codon

   Promoter

   Available since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTECH-MbAcK3RS(IPYE)

   Plasmid

   #104070

   Purpose

   Expression plasmid encoding MbAcK3RS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp MbAcK3RS(IPYE); PproK pyl
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTECH-MmPylRS(IPYE)

   Plasmid

   #104073

   Purpose

   Expression plasmid encoding MmPylRS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp MmPylRS(IPYE); PproK pyl
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTECH-MbPylRS(IPYE)

    Plasmid

    #104072

    Purpose

    Expression plasmid encoding MbPylRS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp MbPylRS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYES2-HTH-HHT2

    Plasmid

    #86482

    Purpose

    yeast expression of HHT2 with His10-TEV-HA tag

    Depositor

    Akash Gunjan

    Article

    Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

    Insert

    HHT2 (HHT2 Budding Yeast)
    

    Use

    Tags

    HIS10-TEV-HA (HTH)

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pTECH-MmAcK3RS(IPYE)

    Plasmid

    #104071

    Purpose

    Expression plasmid encoding MmAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp MmAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTECH-chPylRS(IPYE)

    Plasmid

    #99222

    Purpose

    Expression plasmid encoding chPylRS(IPYE) and pylT

    Depositor

    Dieter Söll

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp chPyl(IPYE)RS; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pyEvolvR (enCas9-PolI5M)

    Plasmid

    #158073

    Purpose

    Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber

    Article

    Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

    Insert

    enCas9-PolI5M
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYES2-Ub-G76V-GFP

    Plasmid

    #11954

    Depositor

    Nico Dantuma

    Article

    Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

    Insert

    Ub-G76V-GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Ubiquitin fused to N-terminus of GFP. Glycine 76 …

    Promoter

    Available since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYeDP60-hATP13A2-E343A

    Plasmid

    #171378

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Use

    Tags

    BAD tag

    Expression

    Yeast

    Mutation

    E343A

    Promoter

    URA3

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYES2-STE2-1D4

    Plasmid

    #133424

    Purpose

    Mutant yeast STE2 coding sequence in vector for expression in yeast S. cerevisiae, with Gal1 promoter.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C59S, C252A

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYES260-sc.eEF2 K509A

    Plasmid

    #85120

    Purpose

    yeast expression of mutant EEF2

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K509A

    Promoter

    GAL1

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

    Plasmid

    #140020

    Purpose

    Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

    Promoter

    EF1

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYES2-gRNA-hyg-MCS

    Plasmid

    #107734

    Purpose

    E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    X-3 site gRNA expression cassettes
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52p

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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