We narrowed to 94 results for: allelic exchange

1. pXY1

   Plasmid

   #90487

   Purpose

   Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

   Depositor

   Sheng Yang

   Article

   Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIMP1-Padc

   Plasmid

   #90486

   Purpose

   Plasmid with adc(CA_P0165) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

   Depositor

   Sheng Yang

   Article

   Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVsc.U6-sgGTTG.CMV/CB-Gluc

   Plasmid

   #121512

   Purpose

   Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   sgGTTG
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEx-deletion-erm

   Plasmid

   #172213

   Purpose

   For allelic exchange in erythromycin-sensitive Prevotella copri strains

   Depositor

   Till Strowig

   Article

   Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

   Type

   Empty backbone

   Use

   Allelic exchange in prevotella copri

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEx-deletion-tet

   Plasmid

   #172214

   Purpose

   For allelic exchange in tetracyclin-sensitive Prevotella copri strains

   Depositor

   Till Strowig

   Article

   Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

   Type

   Empty backbone

   Use

   Allelic exchange in prevotella copri

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.pU1a-SpCas9

   Plasmid

   #121507

   Purpose

   Expresses codon-optimized SpCas9 in mammalian cells.

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   SpCas9
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVsc.U6-sgFah.CMV/CB-EGFP

   Plasmid

   #121508

   Purpose

   Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   sgFah
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP

   Plasmid

   #121509

   Purpose

   Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   sgFah.intron9
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVsc.U6-sgIdua.CMV/CB-EGFP

   Plasmid

   #121511

   Purpose

   Expresses sgRNA targeting mouse Idua intron 9 (sgIdua).

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   sgIdua
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAVsc.U6-sgAspa.CMV/CB-EGFP

    Plasmid

    #121510

    Purpose

    Expresses sgRNA targeting mouse Aspa intron 2 (sgAspa).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgAspa
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLGB30

    Plasmid

    #126620

    Purpose

    Suicide vector for allelic replacement in Bacteroides, tetracycline selection and rhamnose-inducible ss-Bfe1 counterselection

    Depositor

    Laurie Comstock

    Article

    Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

    Type

    Empty backbone

    Use

    Allellic exchange in bacteria

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLGB31

    Plasmid

    #126621

    Purpose

    Suicide vector for allelic replacement in Bacteroides, inulin selection and rhamnose-inducible ss-Bfe1 counterselection

    Depositor

    Laurie Comstock

    Article

    Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

    Type

    Empty backbone

    Use

    Allellic exchange in bacteria

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLGB29

    Plasmid

    #126619

    Purpose

    Suicide vector for allelic replacement in Bacteroides, inulin selection and aTC-inducible ss-Bfe1 counterselection

    Depositor

    Laurie Comstock

    Article

    Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

    Type

    Empty backbone

    Use

    Allellic exchange in bacteria

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19, containing H2-Kb c57/BL6 WK9

    Plasmid

    #102641

    Purpose

    Encodes sequence of the murine H2-Kb allele

    Depositor

    Sai Reddy

    Article

    Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

    Insert

    H2-Kd Balb/c
    

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLGB13

    Plasmid

    #126618

    Purpose

    Suicide vector for allelic replacement in Bacteroides, erythromycin selection and aTC-inducible ss-Bfe1 counterselection

    Depositor

    Laurie Comstock

    Article

    Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

    Type

    Empty backbone

    Use

    Allellic exchange in bacteria

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19, containing H2-Kd Balb/c WK10

    Plasmid

    #102642

    Purpose

    Encodes sequence of the murine H2-Kd allele

    Depositor

    Sai Reddy

    Article

    Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

    Insert

    H2-Kd Balb/c
    

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFW11-ffluc

    Plasmid

    #128487

    Purpose

    Allelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imaging

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    firefly luciferase gene
    

    Use

    Allelic replacement vector

    Tags

    spy0535 downstream flanking region and spy0535 up…

    Promoter

    synthetic

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJQ200mp19

    Plasmid

    #78501

    Purpose

    Suicide vector for allelic exchange

    Depositor

    Michael Hynes

    Article

    Quandt et al Gene. 1993 May 15;127(1):15-21.

    Type

    Empty backbone

    Use

    Suicide vector for gene replacement

    Promoter

    none

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pIMAY*

    Plasmid

    #121441

    Purpose

    Allelic exchange plasmid with PheS* counter-selection for Staphylococcus aureus

    Depositor

    Angelika Grundling

    Article

    Schuster et al Microbiology. 2019 Apr 3. doi: 10.1099/m

    Type

    Empty backbone

    Use

    Escherichia coli/staphylococcus aureus shuttle ve…

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSKAP_AT

    Plasmid

    #122722

    Purpose

    pSKAP plasmid containing the unique DNA barcode "AT" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

    Depositor

    Travis Bourret

    Article

    Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

    Insert

    Barcode AT
    

    Expression

    Bacterial

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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