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pLJM1-zeo G226A E321K ORF29

Plasmid

#200026

Purpose

Lentiviral vector for expression of KSHV ORF29 with G226A and E321K mutation

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF29

Use

Lentiviral

Tags

Expression

Mutation

Glycine 226 changed to alanine, glutamic acid 321…

Promoter

CMV

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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p8.9 NdeltaSB D185K/D186L

Plasmid

#132931

Purpose

p8.9 NdeltaSB bearing RT-D185K/D186L mutant reverse transcriptase sequence

Depositor

Jeremy Luban

Article

De Iaco et al Retrovirology. 2011 Dec 6;8:98. doi: 10.

Insert

gag and pol D185K/D186L

Use

Lentiviral

Tags

Expression

Mutation

D185K/D186L

Promoter

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCW57-mcGAS

Plasmid

#185458

Purpose

Inducible FLAG-tagged mouse cGAS

Depositor

Shitao Li

Article

Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

Insert

cGAS

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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NL4-3Δ6-drEGFP-IRES-mThy1.2

Plasmid

#207881

Purpose

HIV reporter vector expressing dGFP and mThy1.2 from Env locus

Depositor

Edward Browne

Article

Peterson et al Cell Chem Biol. 2023 Dec 21;30(12):1617-

Insert

destabilized EGFP-IRES-mThy1.2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

5' lentiviral LTR

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

Plasmid

#188703

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-EGFP-NLS hygro

Plasmid

#87781

Purpose

Inducible expression of EGFP with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

eGFP

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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HI.fate.SFFV-E

Plasmid

#191555

Purpose

Monitor HIV-1 fate

Depositor

Joseph Sodroski, Alon Herschhorn

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Tags

Expression

Mutation

env-deleted + vpr mutation(s)

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HI.fate.E

Plasmid

#191553

Purpose

Monitor HIV-1 fate

Depositor

Joseph Sodroski, Alon Herschhorn

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Tags

Expression

Mutation

env-deleted + vpr mutation(s)

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HI.fate.UB

Plasmid

#191556

Purpose

Monitor HIV-1 fate

Depositor

Joseph Sodroski, Alon Herschhorn

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Tags

Expression

Mutation

env-deleted + vpr mutation(s)

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HI.fate.SFFV

Plasmid

#191554

Purpose

Monitor HIV-1 fate

Depositor

Joseph Sodroski, Alon Herschhorn

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Tags

Expression

Mutation

env-deleted + vpr mutation(s)

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57-mcGAS-LL/RK-NLS

Plasmid

#185457

Purpose

Inducible nuclear cGAS

Depositor

Shitao Li

Article

Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

Insert

cGAS

Use

Lentiviral

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

L157R, L159K

Promoter

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

Plasmid

#188704

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNAs

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTFG005_Spike_Inducible_3xFlag

Plasmid

#191578

Purpose

Inducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Use

Lentiviral

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pJJH1320

Plasmid

#36915

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

Cre recombinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pJJH1260

Plasmid

#36913

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Inserts

ScURA3
ScURA3

Use

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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pCW57-mcGAS-LL/RK-NLS/D307A

Plasmid

#185459

Purpose

Inducible nuclear cGAS with D307A mutation

Depositor

Shitao Li

Article

Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

Insert

cGAS

Use

Lentiviral

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

L157R, L159K, D307A

Promoter

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-MKK4-EE-NLS hygro

Plasmid

#87779

Purpose

Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only
pLX302-NIPP1-V5 puro

Plasmid

#87775

Purpose

Expresses V5 tagged human NIPP1

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

NIPP1 (PPP1R8 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-P2A-Puro_ST-sgRNA

Plasmid

#188705

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting loci

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

ST sgRNAs

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJJH1295

Plasmid

#36914

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

3x mCherry

Use

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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